Trichoderma-Arabidopsis interaction by Ambient Ionization Mass Spectrometry

Interaction between the plant Arabidopsis thaliana and the fungus Trichoderma atroviride.

Three sample groups (Arabidopsis thaliana, Trichoderma atroviride and Trichoderma-Arabidopsis interaction) with 3 biological replicates were monitored for 10 days, resulting in 40 points. This experiment was focused on monitoring the kinetics of the volatile organic compound (VOC) 6-Pentyl-2H-Pyran-2-One (6-PP), a molecule produced by Trichoderma that helps to establish interaction with plants.  More details and results are available in the publication written by Torres-Ortega et al. (DOI: 10.3390/metabo12121231). 

Data were collected with custom 3D-printed Low-Temperature Plasma (DOI: 10.1021/acs.analchem.6b01019) ambient ionization source, coupled to an LCQ-fleet ion trap (Thermo Scientific, USA). Mass spectra were taken in full spectra at 50-500 m/z range, on profile mode. Each MS scan consists of 10 micros cans. Data was saved under the RAW property format of Thermo. To convert to mzML open format, Proteowizard was used.