Published February 12, 2023 | Version v1
Dataset Open

Imaging dataset 01 for mtFociCounter

Authors/Creators

  • 1. MRC MBU Cambridge

Description

This is the first dataset associated with the updated manuscript on mtFociCounter.

Images are Spinning Disk Confocal Images of unsorted 3t3 NIH mouse fibroblasts stably expressing mitochondrially targeted dsRed, immunofluorescence against dsDNA (mitochondrial nucleoids) and AlexaFluor 647, and stained nuclei with Hoechst.

It contains all data necessary to reproduce the analysis of 3t3 WT sampling (sample-to-sample comparison), as described in the manuscript. "samples" from the same day are 13mm coverslips processed in parallel (seeding of cells, fixation, immunofluorescence) and mounted on the same glass slide (#1 on left, #2 centre, #3 right). The dataset contains the 3t3 WT data from the following acquisition dates:

20220603_sample2
20220603_sample3
20220706_sample2
20220706_sample3
20220708_sample1
20220708_sample2
20220708_sample3
20221022_sample2
20221022_sample3

For details of the sampling procedure, please refer to the accompanying manuscript, which will soon be made available on BioRxiv.
 

Files

01_04_WT_sampling.zip

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