Experimental 3D convex hull tiling of a wild moss specimen ( Rhytidiadelphus or Eurhynchium ?) embedded in 1% agarose
Authors/Creators
Description
Experimental 3D convex hull tiling of a wild moss specimen ( Rhytidiadelphus or Eurhynchium ?) embedded in 1% agarose
Raw data consists of 18 tiles of varying number of Z stacks, depending on where the 3D convex hull points were determined.
Images acquired on a Zeiss Lightsheet Z1 using a W Plan-Apochromat 20x/1.0 UV-VIS objective simultaneous dual camera acquisition of autofluorescence under 488nm and 561nm excitation.
Original voxel sizes are (x,y,z) = (0.326, 0.326, 2.0) microns
Data provided here are tiles fused using BigStitcher, downsampled to the following voxel sizes
- Moss_Fused_Downsampled.tif: (4, 4, 24) microns
- Moss_Fused_Downsampled_Anisotropy_2.tif: (12, 12, 24) microns
Sample acquisition, sample preparation, imaging and stitching performed by Olivier Burri, at the Ecole Polytechnique Fédérale de Lausanne BioImaging and Optics Platform (EPFL - SV - PTECH - PTBIOP)