Published February 3, 2023 | Version 1.2

Sequencing summaries & Nanopolish eventalign outputs for downstream analysis of yeast RNA and synthetic oligonucleotides

Authors/Creators

  • 1. University of Colorado Medical School

Contributors

Supervisor:

  • 1. University of Colorado School of Medicine

Description

This dataset contains:

  1. A set of text files from running the tool Nanopolish eventalign on several nanopore direct RNA sequencing data sets produced by Jay Hesselberth's lab at the University of Colorado (BioProject accession number PRJNA910992), as well as external sequencing data sets from PMID: 34893601 (synthetic oligonucleotides from Leger et al) and PMID: 35252946 (yeast rRNA data from Stephenson et al).
  2. "Sequencing summary" files produced by MinKNOW, from nanopore sequencing of yeast mRNA and synthetic RNA oligos in the Hesselberth lab. We analyze these files in an associated manuscript to determine the "end status" of each read during the sequencing run.

These files can be used as inputs to the R markdown documents at https://github.com/hesselberthlab/RNARePore to reproduce the figures in the associated manuscript.

Files

18S.1428.ivt.eventalign.subset.txt

Files (9.0 GB)

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Additional details