Alzheimer's Disease Prevention through Natural Compounds: Cell- Free, In Vitro, and In Vivo Dissection of Hop (Humulus lupulus L.) Multitarget Activity

The relevant social and economic costs associated
with aging and neurodegenerative diseases, particularly Alzheimer’s
disease (AD), entail considerable efforts to develop effective
preventive and therapeutic strategies. The search for natural
compounds, whose intake through diet can help prevent the main
biochemical mechanisms responsible for AD onset, led us to screen
hops, one of the main ingredients of beer. To explore the chemical
variability of hops, we characterized four hop varieties, i.e., Cascade,
Saaz, Tettnang, and Summit. We investigated the potential
multitarget hop activity, in particular its ability to hinder Aβ1−
42 peptide aggregation and cytotoxicity, its antioxidant properties,
and its ability to enhance autophagy, promoting the clearance of
misfolded and aggregated proteins in a human neuroblastoma SH-SY5Y cell line. Moreover, we provided evidence of in vivo hop
efficacy using the transgenic CL2006Caenorhabditis elegans strain expressing the Aβ3−42 peptide. By combining cell-free and in vitro
assays with nuclear magnetic resonance (NMR) and MS-based metabolomics, NMR molecular recognition studies, and atomic force
microscopy, we identified feruloyl and p-coumaroylquinic acids flavan-3-ol glycosides and procyanidins as the main anti-Aβ
components of hop.