Published January 24, 2023 | Version v1
Dataset Restricted

Supplementary processed data for Chandler et al. Single-cell transcriptomics identifies aberrant glomerular angiogenic signalling in the early stages of a murine model of WT1 kidney disease

Authors/Creators

  • 1. Dr
  • 2. Professor

Description

Summary datafiles for Chandler et al.

  • Processed single-cell RNA sequencing data, derived from murine glomeruli (isolated using the dynabead technique) from n = 2 wild-type Wt1+/+ (Ctrl) and n = 2 mutant Wt1R394W/+ (Mut) littermates of WT1 glomerulopathy, followed by 10x Genomics Chromium v3 platform.
  • Please refer to associated scripts for analysis of the data: https://github.com/daniyal-jafree1995/collaborations/blob/main/Chandleretal_2022_WT1glomerulopathyscRNAseq.R 
  • File descriptions as below:
    • barcodes.tsv.gz - barcodes file required for input to Read10X function in Seurat
    • features.tsv.gz - features file required for input to Read10X function in Seurat
    • matrix.mtx.gz - matrix file required for input to Read10X function in Seurat
    • WT1_scRNAseq.rds - RDS file containing processed and annotated Seurat object for downstream analysis

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