Simultaneous Resolution of Clopidogrel Bisulphate and Aspirin in their Combined Dosage Form by Ratio First Derivative Spectrophotometry

In the present work, the ratio spectra first derivative
spectrophotometry is proposed for the resolution of Clopidogrel
bisulphate (CLP) and Apirin (ASP) in synthetic mixtures prepared in
random fashion and tablets available in 1:1 and 1:2 ratio. The
calibration graph follows Beer’s law in the range of 6 to 30 μcg/ml
for clopidogrel bisulphate and from 4-20 μcg/ml for aspirin. The
mean recoveries & relative standard deviations were found as
99.40% & 0.442% for CLP and 100.09% and 0.905% for ASP,
respectively in 1:1 ratio. Similarly the mean recoveries & relative
standard deviations were found as 999.84% & 1.024% for CLP and
99.95% and 0.578% for ASP, respectively in 1:2 ratio. The graphical
treatment of the overlapping spectra depends on division of the
absorption spectrum of the binary mixture by a standard spectrum of
one of the components and then calculating the first derivative of the
ratio spectrum. These approaches were successfully applied to
quantify CLP combined with ASP in tablets respectively and
validated accordingly for random ratios in synthetic mixtures.