Histopathological Evaluation of Calcium Hydroxide Mixed with Gingerols Versus Metapexas Obturating Material in Treatment of Infected Pulp in Primary Teeth.: Experimental Study

Use of Gingerols in pulp treatment may lead to successful pulp treatment by reducing the body's exposure to chemicals and their potential toxicity. This experimental study was performed to compare the effectiveness of Gingerols versus Metapex as obturating material in the treatment of infected pulp in primary premolar teeth of puppies. Gingerols were extracted and mixed with calcium hydroxide to produce Ginge-Cal. 48 primary premolars of 4 male mongrel puppies aged 4-8 weeks old were selected and divided into four main groups according to the applied root canal filling material. The groups were BL group (Baseline group with normal teeth without intervention), INF (pathological group still without treatment), Ginge-Calexperimental group (root canal filling by calcium hydroxide mixed with Gingerols), and control group Metapex (root canal filling by Metapex). Both groups Ginge-Cal and Metapex divided into two subgroups according to treatment intervals of 4 weeks and 8 weeks (Ginge-Cal4, Ginge-Cal8, Metapex4, and Metapex8). Two puppies were sacrificed after 4 weeks and the other two after 8 weeks. Histological evaluation was done for inflammatory reaction at the apical area for each group. The histopathological results evaluated inflammatory reaction, bacterial colonies, bone destruction, bone remodeling, and periodontal ligament (PDL) thickness. The statistical results showed that the two materials had a positive effect in treating infected pulp teeth (p<0.001), with the superiority of Ginge-Cal, especially at the end of the second period at 8 weeks in a number of comparison points (p<0.001). Depending on histopathological finding Ginge-Cal has the best result than Metapex. This makes it a promising material in the treatment of infected pulp.