Traction force microscopy – Measuring the forces exerted by cells

Cells generate mechanical forces (traction forces, TFs) while interacting with the extracellular matrix or neighboring cells. Forces are generated by both cells and extracellular matrix (ECM) and transmitted within the cell-ECM or cell-cell contacts involving focal adhesions or adherens junctions. Within more than two decades, substantial progress has been achieved in techniques that measure TFs. One of the techniques is traction force microscopy (TFM). This review discusses the TFM and its advances in measuring TFs exerted by cells (single cells and multicellular systems) at cell-ECM and cell-cell junctional intracellular interfaces. The answers to how cells sense, adapt, and respond to mechanical forces unravel their role in controlling and regulating cell behavior in normal and pathological conditions.