Published January 13, 2023
| Version v1
Dataset
Open
Multiplexed histology of COVID-19 post-mortem lung samples - CHRONIC CASE 3 FOV2
Authors/Creators
- 1. Deutsches Rheuma-Forschungszentrum & Charité-Universitätsmedizin Berlin
- 2. Charité-Universitätsmedizin Berlin
Description
Image-based data set of a post-mortem lung sample from a COVID-19 donor (CHRONIC CASE 3 FOV2)
Each image shows the same field of view (FOV), sequentially stained with the depicted fluorescence-labelled antibodies, including surface proteins, intracellular proteins and transcription factors. Images contain 2024 x 2024 pixels and are generated using an inverted wide-field fluorescence microscope with a 20x objective, a lateral resolution of 325 nm and an axial resolution above 5 µm. Images have been normalized and intensities adjusted.
Notes
Funding: Deutsche Forschungsgemeinschaft HA5354/10-1, SPP1937 (HA5354/8-2) and TRR130 P17 and C01 (to Anja Hauser)
Files
CD11b.tif
Files
(385.9 MB)
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Additional details
References
- Mothes, R. and Pascual-Reguant, A. et al. Local CCL18 and CCL21 expand lung fibrovascular niches and recruit lymphocytes, leading to tertiary lymphoid structure formation in prolonged COVID-19. medRxiv 2022.03.24.22272768 (2022) doi:10.1101/2022.03.24.22272768.