Interspecific ICSI for the Assessment of Sperm DNA Damage: Technology Report.

The long-term storage of biological material (sperm, oocytes, embryos, etc.) is essential not only for animal breeding programs, human ART, and basic biology, but also for rescuing endangered animal species and other technologies. Whilst sperm storage is almost perfected for some species (bovine, human, mouse), many problems remain in others. In our contribution, we present a simple approach that can be used for the rapid evaluation of DNA damage level in sperm nuclei after freezing. This approach can be useful especially in those cases when the amount of frozen biological material is limited and permits much higher flexibility when modifying chosen preservation approaches.