Using CRISPR Home Kit to Make a Genome Mutation in E. coli DH5a Bacteria DH5-Alpha Cells are E. coli Cells Engineered to Maximize Variation Efficiency

The CRISPR/Cas9 system has reformed genome editing and has become extensively offered through home kits such as the one used in this research. This study meant to use the CRISPR home kit to make a genome mutation to the rpsL gene in E. coli DH5a bacteria, altering the 43rd amino acid from a Lysine to a Threonine. This alteration would allow the bacteria to survive on Streptococcic media, which would normally prevent its growth. The CRISPR home kit used in this experiment confined all obligatory ingredients, including Cas9, gRNA, and template DNA, as well as tools for testing at home.