Published January 10, 2023
| Version v1
Dataset
Open
Role of eEF2 diphthamide modification in translational fidelity
Description
Supplementary data for the paper : eEF2 Diphthamide Modification Restrains Spurious Frameshifting to Maintain Translational Fidelity
In vitro reconstituted elongation assay (MFFFK-invitro.tif), synthetic growth phenotype of REA1 (MDN1) and dph2 deletion mutant (Rea1-dph2.jpg), Western blot to detect full length Mdn1 and Dyn1 (Western_Mdn1.jpg), growth spot assay for WT (DPH2) and dph2-del (WT-dph2-growth.png), and a script for calculating ribosomal drop-off rate from wig file (drop_off_wig.R).
Files
MFFFK-invitro.tif
Files
(20.6 MB)
| Name | Size | |
|---|---|---|
|
md5:f0d5ff0d2aaa98a46a1bfaea8cc82014
|
14.5 kB | Download |
|
md5:8cb1d4ede75805837204d0d9390ab530
|
18.0 MB | Preview Download |
|
md5:85b1201260171b1fa07928252407604d
|
776.9 kB | Preview Download |
|
md5:5e86e509adc5cb4a4af25f5ec1c47063
|
112.7 kB | Preview Download |
|
md5:92f750b8db529b3b64dcab22b367ebf2
|
1.6 MB | Preview Download |