Published January 6, 2023 | Version v1

Confocal Microscopy Imaging of Peptidoglycan Uptake in the Mouse Intestine

  • 1. Institut Pasteur
  • 2. INSERM
  • 3. Institut Pasteur, INSERM

Description

Confocal imaging data set accompanying Fig.5 and Fig.6 for the article: Wheeler R, Dias Bastos PA, Disson O, Rifflet A, Gabanyi I, Spielbauer J, Bérard M, Lecuit M, Gomperts Boneca I. Microbiota-induced active translocation of peptidoglycan across the intestinal barrier dictates its within-host dissemination (2023) PNAS; doi: 10.1073/pnas.2209936120

Stainings are described in detail in the the PNAS article methodology.
Files names beginning Fig 5A-1; DAPI, WGA, anti-E-cadherin, MDP-rhodamine
Files names beginning Fig 5A-2, Fig 5A-3 & Fig S5;  DAPI, Phalloidin, anti-Siglec-F, MDP-rhodamine
Files names beginning Fig 5B; DAPI, Phalloidin, E.coli peptidoglycan-Alexa Fluor 647 conjugate
Files names beginning Fig 6B; DAPI, Phalloidin, anti-Siglec-F, MDP-rhodamine (also corresponds to Supplementary data Figure 5)
Files names beginning Fig S5-1; DAPI, Phalloidin, anti-CgA, MDP-rhodamine
Files names beginning Fig S5-2; DAPI, Phalloidin, anti-NKM-16-4-2, MDP-rhodamine

Notes

Files may be opened using open source software such as imageJ.

Files

Files (16.9 GB)

Name Size
md5:28e768813967af5d0f537753fc29706e
626.4 MB Download
md5:2f7bb8f79ca8915b9793de6ce8317116
189.1 MB Download
md5:ea478bdf8dbc50d2e60d520371eb736e
61.9 MB Download
md5:379706ce55245130df2135c420df86cd
25.6 MB Download
md5:29a1c7d3a6fbf585c2f0277feeef465c
2.2 GB Download
md5:f2809fd405258996ec962db9059bf9ed
25.7 MB Download
md5:c6bf9556c82056b1daf1fffd3faa1e27
5.2 GB Download
md5:722a29e245026dc4f72264fdbc55f4c8
3.3 GB Download
md5:981f487119eefc3e3107b05180023e1c
2.8 GB Download
md5:22baf00e47845c656523062bb4cea2f8
1.2 GB Download
md5:278ef7f59a5863ae0c2c28dc8e0a7cca
457.2 MB Download
md5:53aaf52fd18c8f01cac2524373fe8f62
506.6 MB Download
md5:11a990ba79e5ff5e6cad1a359c7a9284
115.7 MB Download
md5:32c39142aa49873fa89a3c450105c708
115.7 MB Download

Additional details

Related works

Is supplement to
Journal article: 10.1073/pnas.2209936120 (DOI)