Source data for "Regulation of replication origin licensing by ORC phosphorylation reveals a two-step mechanism for Mcm2-7 ring closing"

Source data for "Regulation of replication origin licensing by ORC phosphorylation reveals a two-step mechanism for Mcm2-7 ring closing" 

The data is organized by Figure and associated Supp Figure(s). A README file is included in each figure folder to explain the files.

(note: Data is included for Figs2-7 and SuppFigs 2-8. Fig.1 and SuppFig.1 did not have any associated data matrices, so there is no upload for them here). 

Briefly, the single molecule data is included in several different formats, all generated from single-molecule TIRF-microscopy video files using Matlab:

Integrated trace files "traces" include integrated fluorescence intensity at individual DNA molecules over a 20 minute reaction.

Background-corrected trace files normalize the integrated fluorescence intensity to a local-average background, as described in the Methods section of the paper-- these are used for EFRET calculations.

"Intervals" files include the start, end time, and duration of protein-DNA interactions and were generated from the trace files and the raw videos using the Imscroll program (available at: https://github.com/gelles-brandeis/CoSMoS_Analysis).

EFRET vectors are concatenated vectors of the EFRET vs time values of all protein-DNA interactions in the experiment-- these are used to generate EFRET heat maps. 

Matlab analysis scripts used in the paper are uploaded in a separate folder "ALA_scripts_used_final". Several of these scripts reference custom Matlab functions from the Gelles lab which are available here: https://github.com/gelles-brandeis/jganalyze and should be downloaded along with my attached scripts file. 

note: Raw single-molecule video files have not been uploaded here due to large sizes, but can be provided upon request.