Supplementary materials. Publication "Three-way relationships between gut microbiota, helminth assemblages and bacterial infections in wild rodent populations" by Bouilloud et al.

Supplementary information

Supplementary Figure S1. Maps showing the sampling area (left) and localities (right) in France. Forests are indicated in green and water in blue. The four sampling localities are represented with a colored polygon. The arrow indicates the North.

Supplementary Figure S2. Composition of the gut bacteriota. The relative abundance of six phyla representing 99% of the total composition is represented. Individuals are grouped by sampling localities, which are ordered from North to South. (A) Bar graph shows individual variation in phyla composition (phylum=color). (B) Box and whisker plots represent median and interquartile values for each phylum. Black dots correspond to mean values, and colored dots correspond to individuals.

Supplementary Figure S3. Variations of alpha diversity with individual factors, for the gut bacteriota (family level), pathogenic bacteria and gastro-intestinal helminths of bank voles. Alpha diversity is estimated using the specific richness (A, B and C) and the Shannon index (D, E and F). In graphs C and F, the blue line corresponds to the linear regression line.

Supplementary Figure S4. Relationships between the composition of the gut bacteriota, pathogenic bacteria and gastro-intestinal helminth communities: The db-RDA triplot shows the structure of the gut bacteriota at the phylum level and the correlations with the intra-host parasite communities. The arrows correspond to the significant explanatory variables. Each point corresponds to an individual, and the colors correspond to the different sampling localities.

Supplementary Table S1. Variation of the Firmicutes/Bacteroidetes ratio with localities and individual factors.

Supplementary Table S2. Alpha diversity metrics and statistics for the gut bacteriota, pathogenic bacteria and helminth communities of bank voles.

Supplementary Table S3. Beta diversity metrics and statistics for the gut bacteriota, pathogenic bacteria and helminth communities of bank voles