In vitro Bioaccumulation of Heavy Metals by Water Lettuce Pistia stratiotes Engl (Araceae) and its Combustion Process as Manure Value by SEM-EDX Analysis

ABSTRACT

In vitro experiments on chromium, copper, lead and zinc heavy metals bioaccumulation using water lettuce Pistia stratiotes Engl (Araceae) was conducted with 5, 10 and 20 mg/100 ml concentration for a period of 20 days. The SEM-EDX was used to characterize the interaction between the metal and plant. The results revealed the bioaccumulation of lead as high as 37.79% followed by chromium 8.63%, zinc 6.00% and copper 2.37%. The combustion process of bioaccumulated P. stratiotes biomass have shown the total reduction of lead 3.84%, copper 2.34%, zinc 1.26% and chromium 0.99%. The combusted biomass in the form of ash 10% and river sand passed through 1 mm sieve, sterilized and was amended to Pennisetum typhoides pot culture studies, revealed the healthy growth, ensure the manure value of metal-loaded biomass.

Keywords:   Bioaccumulation, combustion process, heavy metal, manure value,  Pistia stratiotes, SEM-ED

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