Immobilization of α-Amylase by Pseudomonas sp isolated from Indian Chicken

ABSTRACT

Control of α-Amylase formed by a Pseudomonas sp isolated from Indian Chicken feather was deliberate. Partly cleansed enzyme with 486.77 IU and specific activity of 811.28 units mg (protein) was used for immobilization study. An easily obtainable and cheap 3% Sodium alginate matrix with easy control gel entrapment procedure was used for tricking the enzyme showed 555.5 IU activity. The optimization was carried out to study the catalytic properties which showed the optimum pH, temperature and substrate concentration at pH 7, 450C and 16mg, respectively. The reusability of the arrested enzyme preparation presented its use in incessant starch hydrolysis for up to 10 cycles. This immobilized enzyme can be used as a standby of profitable enzyme since it has shown same greater operational flexibility and enzymatic activity of the pure enzyme.       

Keywords: Immobilization, Calcium alginate, Immobilization, Pseudomonas sp.

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