Statistically unbiased prediction enables accurate denoising of voltage imaging data
- 1. School of Electrical Engineering, Korea Advanced Institute of Science and Technology, Daejeon, Republic of Korea
- 2. Department of Materials Science and Engineering, Korea Advanced Institute of Science and Technology, Daejeon, Korea
- 3. Department of Chemistry and Chemical Biology, Harvard University, Cambridge, United States
- 4. Department of Biology, Chungnam National University, Daejeon, South Korea
- 5. School of Biological Sciences, Seoul National University, Seoul, Republic of Korea.
- 6. Allen Institute for Neural Dynamics, Seattle, WA, USA.
Description
Here we report SUPPORT (Statistically Unbiased Prediction utilizing sPatiOtempoRal information in imaging daTa), a self-supervised learning method for removing Poisson-Gaussian noise in voltage imaging data. SUPPORT is based on the insight that a pixel value in voltage imaging data is highly dependent on its spatially neighboring pixels in the same time frame, even when its temporally adjacent frames do not provide useful information for statistical prediction. Such spatiotemporal dependency is captured and utilized to accurately denoise voltage imaging data in which the existence of the action potential in a time frame cannot be inferred by the information in other frames. Through simulation and experiments, we show that SUPPORT enables precise denoising of voltage imaging data while preserving the underlying dynamics in the scene.
Datasets for volumetric structural imaging of penicillium and calcium imaging of zebrafish.
Volumetric structural imaging of penicillium
221110_C2_16X0p8NA_w_Pen_FITC_0p5Hz_SinglePlane_S1R1_xy0p34um_laser0p5_Gain10_pinhole2p7AU.tif
--> Low SNR image
221110_C2_16X0p8NA_w_Pen_FITC_0p5Hz_SinglePlane_S1R2_xy0p34um_laser10_Gain2_pinhole2p7AU.tif
--> High SNR image
Calcium imaging of zebrafish
221024_C2_16X0p8NA_w_casper_GCaMP7a_4dpf_FITC_1Hz_SinglePlane_Whole_S7R1_xy0p75um_laser1_Gain10_pinhole1p8AU.tif
--> Multiple regions
221024_C2_16X0p8NA_w_casper_GCaMP7a_4dpf_FITC_2Hz_SinglePlane_Ce_S7R1_xy0p34um_laser1p5_Gain10_pinhole1p8AU.tif
--> Cerebellar plate
221024_C2_16X0p8NA_w_casper_GCaMP7a_4dpf_FITC_2Hz_SinglePlane_D_S7R1_xy0p34um_laser1p5_Gain10_pinhole1p8AU.tif
--> Dorsal telencephalon
221024_C2_16X0p8NA_w_casper_GCaMP7a_4dpf_FITC_2Hz_SinglePlane_MO_S7R1_xy0p34um_laser1p5_Gain10_pinhole1p8AU.tif
--> Medullar oblongata
221024_C2_16X0p8NA_w_casper_GCaMP7a_4dpf_FITC_2Hz_SinglePlane_OB_S7R1_xy0p34um_laser5_Gain20_pinhole1p8AU.tif
--> Olfactory bulb
221024_C2_16X0p8NA_w_casper_GCaMP7a_4dpf_FITC_2Hz_SinglePlane_OT_S7R1_xy0p34um_laser1p5_Gain10_pinhole1p8AU.tif
--> Optic tectum
221024_C2_16X0p8NA_w_casper_GCaMP7a_4dpf_FITC_4Hz_SinglePlane_Hb_S7R1_xy0p34um_laser1p5_Gain15_pinhole1p8AU.tif
--> Habenula