Protective effect of Immunex ds against Hepatitis B in a mouse experimental model

ABSTRACT

Heart DNA was estimated in immunostimulated, immunostimulated + vaccine treated and normal male swiss albinomice. One group (I) mice treated with immunex DS (IDS) (@ 150mg/mouse); six groups of experimental (A, B, C, D, E and F) were treated with IDS + hepatitis B vaccine and one group (N) was kept as normal’s (untreated with IDS + vaccine). Two mice from all the groups I, N and A to F were sacrificed on day 1, 2, 3, 4 and 5 of experimental period. Experimental mice (groups A to F) which received IDS + vaccine showed an increase of heart DNA when compared with controls (group N) and decrease in comparison with immunostimulated mice (group I).

Key Words: Heart, DNA, Immunostimulant, Hepatitis, Mice. 

REFERENCES

1. Bhatnagar, D., Soran, H. and Durington, P.N. (2008). Hypercholesterolaemia and its maangement. BMJ. 337: a993.
2. Bolli, R. (1991).  Oxygen-derived free radicals and myocardial reperfusion: an overview. Cardiovasc. Drugs. Ther. 5: 249-268.
3. Burton, K. (1956).  A study of the conditions and mechanism of the diphenylamine reaction for the calorimetric estimation of deoxyribonucleic acid. Biochem. J. 62: 315-323.
4. Chayama, K., Hayes, C.N., Hiraga, N., Abe, H., Tsuge, M. and Imamura, M. (2011). Animal model for study of human hepatitis. Viruses. J. Gastroenterol. Hepatol. 26(1): 13-18.
5. Citarasu, T., Sivaram, V., Immanuel, G., Rout, N. and Nurugan, V. (2006).  Influence of seleced indian immunostimulant herbs against white spot syndrome virus (WSSV) infection in black tiger shrimp Penaeus monodon with reference to haematological, biochemial and immunological changes.  Fish. Shellfish Immunol. 21(4): 372-384.
6. Demirci, F., Bayraktaroglu, Z., Karoglan, M., Coskun, Y., Karooglan, K. II. and Okan, V. (2005).  Immunomodulatory effects of HBS Ag Vaccine and levamisole in chronic hepatitis B and hepatitis B carrier children. Turkish J. Gastroenterol. 16(4): 188-193.
7. Divya Teja , S. and Viveka Vardhini, V. (2015). Total protein, DNA and RNA content in the abdominal muscles of mice treated with immune and hepatitis B vaccine. Biolife 3(2):469-475.
8. Gelina, J., Yin, G., Ardo, L. and Jeney, Z. (2009).  The use of immunostimulating herbs in fish. An overview of research. Fish Physiol. Biochem. 35(4): 669-676.
9. Halder, S., Bharal, N., Parmod, K., Mediratta, Inderjeet Kaur and Krishna K. Sharma (2009). Anti-inflammatory, immunomodulatory and antinociceptive activity of Terminalia arjuna. Roxb bark powder in mice and rats. Ind. J. Exp. Biol. 47(7): 577-583.
10. Hearse, D.J. and Bolli, R. (1992). Reperfusion induced injury: manifestations, mechanisms and clinical relevance. Cardiovasc. Res. 26: 101-108.
11. Hearse, D.J. and Tosaki, A. (1987).  Free radiclas and reperfusion induced arrhythmias: Protection by spin trap agent PBN in the rat heart. Circ. Res. 60: 375-383.
12. Jasmin Gold, V. and Viveka Vardhini, V. (2016). Protein level in the heart of mice during immunostimulation and hepatitis infection. Biolife 4(2):271-274.
13. Jin, Z., Sun, R., Wei, H., Gau, X., Chen, Y. and Tian, Z. (2011). Accelerated liver fibrosis in hepatitis B virus transgenic mice: involement of naturla killer T cells. Hepatol. 53(1): 219-229.
14. Kumar, D., Hacker, T.A., Buck, J., Whitesell, L.F., Kaji, E.H., Douglas, P.S. and Kamp, T.J. (2005). Distinct mouse caronary anatomy and myocardial infarction consequent to ligation. Coron. Artery Dis. 16: 41-44.
15. Marinova, S., Tchorbadjiiska, L., Petrunov, B., Cvetanov, J., Nenkov, P., Konstantinova, D. and Markova, R. (2000). Immunostimulating and protective effects of an oral polybacterial immunomodulator ‘Dentavax’ in a rabbit experimental model. Int. J. Immunopharmacol. 22(11): 843-854.
16. Maxwell, S. and Lip, G. (1997).  Reperfusion injury: a review of the pathophysiology clinial manifestations and therapeutic options.  Int. J. Cardiol. 58: 95-117.
17. Mudge, Jr. G.H., Mills, Jr., R.M., Taegtmeyer, H., Gorlin, R. and Lesch, M. (1976) Alterations of myocardial amino acid metabolism in chronic ischemic heart disease. J. Clin. Invest. 58: 1185-1192.
18. Nilles, K.M. and London, B. (2007).  Knockin animal models of inherited arrhythmogenic diseases; what have we learned frm them J. Cardiovasc. Electrophysiol. 18(10): 1117-1125.
19. Park, B., Brinkmann, M.M., Spooner, E., Lee, C.C., Kim, Y.M. and Ploegh, H.L. (2008). Proteolytic cleavage in an endolysosomal compartment is required for activation of toll-like receptor 9. Nat.Immunol. 9: 1407-1414.
20. Petrunov, B. (2004). Poly bacterial immunostimulators in medical practice. J. Microbiol. Epidemiol. Immunobiol. 6: 122-126.
21. Petrunov, B., Nenkov, P and Shekerdjiisky, R. (2007).  The role of immunostimulants in immunotherapy and immunoprophylaxis. Biotechnol. Biotechnol. 21(4): 454-462.
22. Piper, H., Garcia, D.D. and Ovize, M. (1998).  A fresh look at reperfusion injury. Cardiovasc. Res. 38: 291-300.
23. Sakunthala, G., Nathanel, P.J.R. and Vardhani, V.V. (2014). Impact of immunostimulant on stomach protein and DNA activity during hepatitis B infection in mice. Ind. J. Sci. Res. Tech. 2(2): 13-17.
24. Zhan Gao, Feng-Jen-Liu, Li Liu, Tan-You Zhou, Jun Lei., Lu Xu., Cong Liu., Jie Dai., En-Qians Chco and Hong Tang (2010).  Application of hepatitis B virus replication mouse model. World J. Gastroenterol. 28: 16(16): 1979-1985.
25. Zhang, Y., Zhou, J.H., Yin, X.L., and Wang, F.Y. (2010).  Treatment of hepatitis B virus associated glomerulonephritis: a meta analysis. World J. Gastoenterol. 14: 16(6): 770-777.