Fast In-Vitro Regeneration of an Important Medicinal Plant Karaveera (Nerium odorum L.)

ABSTRACT

Nerium odorum, Linn. (Apocynaceae) an important evergreen shrub which is heat, salinity, and drought tolerant.  Plants with milky sap has medicinal value, mainly cardenolides, flavonoids, and terpenes. It comprises of total 01 species. Explants of an alkaloid producing plant cultured in vitro and has been found to retain the capacity to synthesis alkaloids identical to that in the intact plant. It is used for wastewater purification and for restoration of riparian woodlands. In view of these facts the study was conducted for micro propagation of Nerium odorum. MS media supplemented with different concentrations (0. 5–10.0 mg/l) of NAA, 2, 4-D, BAP and KIN was used singly and in combination. Among all the growth hormones, in single combination 2, 4-D was the best for callus induction (75% in stem and 79% in leaf) and in combination 2, 4-D and BAP was found the most suitable for callus induction (78% in stem and 81% in leaf).  Day of callus induction started from 19^th to 37^th day. This variation is due to the difference in culture conditions and the age of explants. In single combination BAP was the best for shooting (75%) and in double combinations BAP (1.5mg/l) and NAA (0.5mg/l) were the best for shooting (81%). Higher induction of root (85%) was observed at NAA (1.5 mg/l) and in double combinations BAP (1.5mg/l) and NAA (0.5mg/l) were the best for rooting (86%). Regenerated plants after hardenings were transferred to soil and they showed 77% survival. The protocol was optimized by manipulations of different plant hormones for enhanced multiplication. Protocol explained here provides a rapid plant regeneration system which could be used for the commercial purposes. 

 Key words: Nerium odorum, callus culture, micro propagation, plant growth regulators.

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