Mechanisms Governing Target Search and Binding Dynamics of Hypoxia-Inducible Factors

Raw fast SPT data for Hypoxia-Inducible Factors:

HIF2a_HIF1b_KIN_Clones.zip corresponds to Figure 2A-C and Figure 2-Figure supplement 2A-B, contains fast SPT data for Halo-HIF-2α KIN clone A31 and clone B50, Halo-HIF-1β KIN clone A21 and clone B89.

HIF1b_with_HIF2a_overexpression.zip corresponds to Figure 2D-E and Figure 2-Figure supplement 2C, contains fast SPT data for: Halo-HIF-1β KIN clone A21, Halo-HIF-1β KIN clone A21 with HIF-2α stably over-expressed, and Halo-HIF-1β KIN clone A21 with a dimerization mutant form of HIF-2α (R171A/V192D) stably over-expressed.

Belzutifan_dosage.zip corresponds to Figure 3-Figure supplement 1, contains fast SPT data for: Halo-HIF-2α KIN clone A31, treated with 0.0002 uM,  0.002 uM, 0.005 uM, 0.01 uM, 0.02 uM, and 0.2 uM of Belzutifan.

HIF1b_with_HIFa_chimera_overexpression_and_Belzutifan.zip corresponds to Figure 3 and Figure 3-Figure supplement 2, contains fast SPT data for: Parental Halo-HIF-1β KIN clone A21 cells and cells stably over-expressing either a certain form of HIF-α (WT or domain swap), treated with either Belzutifan or DMSO. 

HIFa_domain_swap.zip corresponds to Figure 4 and Figure 4-Figure supplement 2, contains fast SPT data for: Halo-HIF-2α KIN clone A31, WT 786-O cells stably express V5-Halo-HIF-2α with an L30 promoter, WT 786-O cells stably express V5-Halo-HIF-2α/1α with an L30 promoter, WT 786-O cells stably express V5-Halo-HIF-1α with an L30 promoter, and WT 786-O cells stably express V5-Halo-HIF-1α/2α with an L30 promoter.  

DBD_mutant.zip corresponds to Figure 5A-C, contains fast SPT data for: WT 786-O cells stably express V5-Halo-HIF-2α with an L30 promoter, WT 786-O cells stably express a DBD mutant form of V5-Halo-HIF-2α (R27E) with an L30 promoter, WT 786-O cells stably express V5-Halo-HIF-1α with an L30 promoter, and WT 786-O cells stably express a DBD mutant form of V5-Halo-HIF-1α (R30E) with an L30 promoter.

Dimerization_mutant.zip corresponds to Figure 5D-F, contains fast SPT data for: WT 786-O cells stably express V5-Halo-HIF-2α with an L30 promoter, WT 786-O cells stably express a dimerization mutant form of V5-Halo-HIF-2α (R171A/V192D) with an L30 promoter, WT 786-O cells stably express V5-Halo-HIF-1α with an L30 promoter, and WT 786-O cells stably express a dimerization mutant form of V5-Halo-HIF-1α (R170A/V191D) with an L30 promoter.