Published November 10, 2022
| Version v1
Preprint
Open
Brain imaging and registration in larval zebrafish
Authors/Creators
- 1. NICHD
Description
Registration of larval zebrafish brain scans to a common reference brain enables comparison of transgene
and gene expression patterns, neuroanatomy, and morphometry. Here we describe step-by-step methods
for staining and mounting larval zebrafish to facilitate whole-brain fluorescence imaging of transgenic
lines, immunohistochemical and dye stains and mRNA localization using hybridization chain reaction.
Following image acquisition, we provide a template for aligning brain images to a reference atlas using
non-linear registration with the ANTs software package.