Anthelmintic activity of Zingiber roseum rhizomes against Pheretima posthuma: In vitro and in silico approach
Authors/Creators
- 1. 1. Department of Pharmacy, Eastern University, Bangladesh
- 2. 2. Department of Biotechnology and Genetic Engineering, Noakhali Science and Technology University
- 3. 3. Department of Pharmacy, Noakhali Science and Technology University
Description
Abstract
Background : The goal of this study was to investigate the anthelmintic activity of a methanol extract of Zingiber roseum rhizome (ZRR) in an experimental model, followed by an in silico molecular docking study and an ADMET analysis.
Methods : A worm called Pheretima posthuma was used to test the anthelmintic activity. Then, molecular docking study was performed of identified compounds of Z.roseum rhizome from HPLC namelycatechin hydrate, epicatechin, trans-ferulic acid, rosmarinic acid,quercetin, myricetin, trans-cinnamic acid having maximum activity against β-TUBULIN enzyme by using CB-Dock protein-ligand docking server. Additionally, DruLiTo software was used to investigate drug-like predictions, and ADME/T profiles were examined using Swiss ADME Analysis and Molinspiration Cheminformatics software.
Results : ZRR contained alkaloids, flavonoids, tannins, and saponins, according to preliminary phytochemical analysis. On an adult worm Pheretima posthuma, ZRR exhibited dose-dependent and statistically significant anthelmintic activityThe best concentration of ZRR for anthelmintic activity on Pheretima posthuma was compared to the reference standard Albendazole (2.5 & 5.0 mg/ml).Our molecular docking study, on the other hand, reveals that the phenolic compounds (except trans-cinnamic acid) have strong binding affinities (ranging from -6.4 to -8.5 KCal/mol) to β-tubulin.These phyto-compounds from natural sources could be used to develop a reliable medication or to aid in lead identification using modern techniques.
Conclusion : The current study's findings confirmed Zingiber roseum rhizome extract's potential anthelmintic activity, and all compounds (except trans-cinnamic acid) were found to be effective in computer-aided drug design models.
Keywords : Zingiber roseum, Tannins, Anthelmintic, Molecular docking, QSAR prediction