GST level in the abdominal muscles of mice treated with Immunex DS and GeneVac B vaccine

ABSTRACT

The effect of immunostimulant, Immunex DS (IDS) and Gen Vac B vaccine was tested against GST in the abdominal muscle of male Swiss albino mice (6 - 8 weeks old; 23 - 26g wt) at different days of experiment. Six groups of experimental mice (A, B, C, D, E and F), one control group (U) and another IDS treated group (I) were selected. IDS was orally administered @ 150mg/mouse on 0 day (group I). To all the mice of groups A to F, IDS was orally administered (@ 150mg/mouse) on 0 day and Gen Vac B vaccine was inoculated on day 4 of experiment (0.07 ml/mouse in group A, 0.1ml/mouse in group B, 0.2ml/mouse in group C, 0.4ml/mouse in group D, 0.8ml/mouse in group E, 1.0ml/mouse in group F). Group U was untreated (with IDS) and uninfected. Two mice from each experimental (A, B, C, D, E and F) and control groups U and I (after day 7 of vaccine treatment in case of experimentals) were necropsied from day 1-5. Abdominal muscle tissue was separated and analyzed for GST activity using standard method. Increased activity of GST might be due to the regulation of active oxygen species which play a significant role in pathogenesis of muscular dystrophies. It is evident that IDS and/or vaccine might have caused stress resulting in the marked alteration in the GST level in the abdominal muscles of mice.

Keywords: GST, Abdominal muscles, Mice, Immunostimulant, Hepatitis B.

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