THE EFFECT OF TREHALOSE, CAFFEINE AND GLUTATHIONE ON BOVINE SPERMATOZOA: 2. MORPHOLOGY AND OXIDATIVE STATUS IN VITRO

The aim of this study was to examine the effects of glutathione, trehalose and caffeine on selected bovine spermatozoa morphology parameters, as well as lipid peroxidation following an in vitro cultivation at different temperatures and time periods. Morphological analysis showed similar occurrence of morphological abnormalities in all group with no significant differences (p>0.05). The TBARS assay revealed that the selected concentrations of trehalose, caffeine and glutathione did not have a significant effect (p>0.05) on lipid peroxidation as a process leading to oxidative stress development in spermatozoa.