Biochemical Study of Some New Cephems and Selenacephems Based on 6H-1,3-Thiazines and 6H-1,3-selenazines

Several new and know 6-(4-substituted phenyl)-4-(4-substituted phenyl)-2-phenyl-6H-1,3-thiazine (or selenazine) (Z4B₇, Z4D₅, Z4B_7' and Z4D_5') were prepared by the 1,4-Michael addition reaction of chalcone derivatives with thiobenzamide or phenylselenocarboxamide in basic medium (where the chalcones was formed by Claisen-Schimidt condensation of aromatic aldehydes with 4-substituted acetophenone in presence of sodium hydroxide).  These 6H-1,3-thia- or selenazine were used to a new series of cephem and selenacephem compounds (i.e. 7-chloro-4-(4-substituted phenyl)-2-(4-substituted phenyl)-6-phenyl-5-thia (or 5-selena)-1-azabicyclo[4.2.0]oct-2-en-8-one; AZ4B₇, AZ4D₅, AZ4B_7' and AZ4D_5'). All new compound derivatives were characterized by IR, ¹H NMR, ¹³C NMR, mass spectroscopic techniques and elemental analysis. The toxicity of new compounds was assayed via the determination of their LD₅₀ value by using Dixon^'s up and down method.  The antibacterial activity of cephem and selenacephem compounds were tested in vitro against Staphylococcus aureus, Bacillus, Escherichia coli and Pseudomonas aeruginosa. Furthermore, the antioxidant, anticancer and DNA cleavage efficiency of compounds were evaluated.