Comparative study of Bombyx mori nucleopolyhedrovirus replication in larval cell lines of Bombyx mori

ABSTRACT

A four new cell lines viz., Bm-1, Bm-12 Bm-16, Bm-17 and Bm -5 is a worldwide used cell line are tested for BmNPV (Bombyx mori nuclepolyhdedrovirus) susceptibility and its replication The BmNPV was serially passaged in the Bm-12, Bm-1, Bm-16, Bm-17 and Bm-5 cell lines for five times. All the cell lines were found susceptible to BmNPV infection when inoculated with the haemolymph of BmNPV infected silkworm larvae. The infection rate was Bm-5 (93.79%), Bm-16 (91.30%), Bm-1 (90.30%), Bm-12 (87.20%) and Bm-17 (80.43%). 

Keywords: Bombyx  mori,  Ovarian  cell  lines,  BmNPV infection

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