Bioremediation potential of textile Aspergillus flavus teak 07 Against textile dye and their toxicity assessment
Authors/Creators
- 1. P.G and Research Department of Biotechnology, National College, Tiruchirappalli-620 00, India
- 2. P.G and Research Department of Microbiology, D.G Vaishnav College, Chennai-600 106,India
Description
ABSTRACT
The study focused on the isolation, decolourization efficiency of the indicator organism and their toxicity assessment. The predominant isolate was morphologically identified, characterized by 18SrRNA and named as Aspergillus flavus TEAK 07. The indicator and the reference fungi Aspergillus flavus MTCC 1883 strain were assessed for their efficacy to decolorize the reactive Red 120. The indicator organism Aspergillus flavus TEAK 07 expressed the best decolorization efficiency than the reference isolate. The various factors affecting Reactive red decolonization were optimized, include temperature, pH, different carbon and nitrogen sources, and different agro substrate. Results showed that pH 5.5 and temperature of 28ºC was optimal. In addition to this, glucose and ammonium were found to be a better carbon source, and nitrogen source, the initial inoculum concentration of 1% and 0.1 mg/ml of initial dye concentration was found to give maximum decolourization. Vigna mungo seed germination tests proved, decolorized dye was less toxic than the original dye.
Keywords: Reactive dye 120, Aspergillus flavus TEAK 07, Decolorization, Seed Germination.
REFERENCES
- Abubacker, M.N., Srinivasan,S and Visvanathan, M. (2013) Invitro bioremediation of Azored dye by indicator fungal isolates Biosciences Biotechnology Research Asia, 10:295 – 300.
- Bor-Yann, C. (2002). Understanding decolorization characteristics of reactive azo dyes by Pseudomonas luteola: toxicity and kinetics. Process Biochem. 38 (3): 437–446.
- Bras R, Ferra IA, Pinheiro HM, (1997) Goncalves IC. Batch tests for assessing decolourisation of azo dyes by methanogenic and mixed cultures. Journal of Biothechnology.89: 155–62.
- Carliell, C.M, Barclay, S. J, Naidoo, N., Buckley, C. A, Muholland, D. A. and Senior, E. (1995). Microbial decolourization of a reactive azo dye under anaerobic conditions. Water SA. 21: 61-69.
- Chang, J. S., Chou, C., Lin, Y. C., Lin, P. J., Ho, J. Y. and Hu, T. L. (2001). Kinetic characteristics of bacterial azo-dye decolorization by Pseudomonas luteola. Wat. Research. 35(12): 2841-2850.
- Chen, K. C., Wu, J. Y., Yang, W. B., and John Hwang, S. C. (2003). Evaluatiion of effective diffusion coefficient and intrinsic kinetic parameters on azo dye degradation using PV A- immobilized cell beads. Biotech.Bioeng., 83: 821-832.
- Chen, K. C., J. Y. Wu, D. J. Liou, and S. C. J. Hwang, (2003)a. Decolorization of the Textile Dyes by Newly Isolated Bacterial Strains. J. Biotechnol., 101: 57- 68.
- Chung, K.T., and C. E. Cerniglia. (1992). Mutagenicity of azo dyes: structure activity relationships. Mutat. Res. 277:201–220.
- Dos Santos, A. B., F. J. Cervantes, and J. B. van Lier. (2004). Azo Dye Reduction by Thermophilic Anaerobic Granular Sludge, and the Impact of the Redox Mediator Anthraquinone- 2,6-disulfonate (AQDS) on the Reductive Biochemical Transformation. Appl. Microbiol. Biotechnol., 64: 62 - 69.
- Flores, Y., Flores, R., Gallegos, A.A. (2008). Heterogeneous Catalysis in the Fenton-type System Reactive Black 5/H2O2. Journal of Molecular Catalysis, 281: 184-191.
- Harley, J.P. & Prescott, L.M., 1993. Laboratory Exercises in Microbiology, 2nd edition. Wm. C Brown Publishers, Iowa.
- Isik, M., Sponza, D.T. (2004). Monitoring of toxicity and intermediates of CI Direct Black 38 azo dye through decolorization in an anaerobic/aerobic sequential reactor system. Journal of Hazardous Materials 114: 29–39.
- Jo-Shu Chang_, Tai-Shin Kuo, Yun-Peng Chao, Jin-Yen Ho & Ping-Jei Lin. (2000). Azo dye decolorization with a mutant Escherichia coli strain. Biotechnology Letters 22: 807–812.
- Kalme, S.D., Parshetti, G.K., Jadhav, S.U., Govindwar, S.P., (2006). Biodegradation of benzidine based dye Direct Blue-6 by Pseudomonas desmolyticum NCIM 2112. Bioresour. Technol. 98: 1405–1410.
- Kalyani D.C, Patil, P.S, Jadhav, J.P, Govindwar, S. (2007). Biodegradation of reactive textile dye Red BLI by an isolate d bacterium Pseudomonas ssp. SUK1. Biores+ur.Technology. 99:4635-4641.
- Kilic, N. K., J. L. Nielsen, M. Yuce, and G. Donmez. (2007). Characterization of a Simple Bacterial Consortium for Effective Treatment of Wastewaters with Reactive Dyes and Cr(VI). Chemosphere, 67: 826-831.
- Kodam, K. M., I. Soojhawon, P. D. Lokhande, and K. R. Gawai. (2005). Microbial Decolorization of Reactive Azo Dyes under Aerobic Conditions. World J. Microbiol. Biotechnol., 21: 367-370.
- Krishna Murari Kumar. Seasonal abundance of Myzus persicae (Sulzer) and its association with food plants and natural enemies in Northeast Bihar, Biolife 2013;1(4);195-199
- Mamidala, E, RP Gujjeti. 2013. Phytochemical and antimicrobial activity of Acmella paniculata plant extracts. J Biol Innov 2 (1), 17-22.
- Panswad, T., Luangdilok, W. (2000). Decolourization of reactive dyes with different molecular structures under different environmental conditions. Water Res. 34: 4177-4184.
- Prashanth, S. Mathivanan, N (2010).Growth promotion of groundnut by IAA Producing rhizobacteria Bacillus licheniformis MML2501.Archives of phytopathology & plant protection, 43 (2) : 191-208.
- Sarioglu, M. and Bisgin, T., (2007). Removal of Maxilon Yellow GL in a mixed methanogenic anaerobic culture. Dyes and Pigments 75: 544–549.
- 21.Sani, R. K. and Banerjee V.C. (1999). Decolorization of Triphenylmethane Dyes and Textile and Dyestuff Effluent by Kurthia sp. Enzyme Microb. Technol., 24: 433-437.
- Pollock, V.V, R.C. Conover, R.C, Johnson, M.J, Barber,M.J (2002). Bacterial expression of the molybdenum domain of assimilate nitrate reductase: production of both the functional molybdenum-containing domain and the nonfunctional tungsten analog. Arch. Biochem. Biophys. 85: 237–248.
- Vijaykumar, M.H., Vaishampayan, P.A., Shouche, Y.S., Karegoudar, T.B. (2007). Decolorization of naphthalene-containing sulfonated azo dyes by Kerstersia sp. strain VKY1. Enzyme and Microbial Technology 40: 204–211.
- Wuhrmann, K., Mechsner, K., Kappeler, T. (1980). Investigation on rate-determining factors in the microbial reduction of azo dyes. Eur J Appl Microbiol. 9: 325-338
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