Published November 30, 2022
| Version v1
Dataset
Open
Fibrin bead assay with VEGF-A stimulation.
- 1. A.I. Virtanen Institute for Molecular Sciences, University of Eastern Finland, Kuopio, Finland
- 2. A.I. Virtanen Institute for Molecular Sciences, University of Eastern Finland, Heart Center, Kuopio University Hospital, Gene Therapy Unit, Kuopio University Hospital, Kuopio, Finland
Description
Confocal microscopy images of fibrin bead assay.
Related article for citation: https://www.nature.com/articles/s41598-023-33090-6 "SproutAngio: an open-source bioimage informatics tool for quantitative analysis of sprouting angiogenesis and lumen space"
Cells: Human umbilical vein endothelial cells (HUVECs) were isolated, maintained in PromoCell Endothelial Cell Growth Medium (Heidelberg, Germany) with 1X endothelial growth kit addition, and used in passage 2. Human lung pulmonary fibroblasts (hPFs) were purchased from PromoCell, maintained in DMEM with 10% FBS, and used in passage 8.
Experimental groups with VEGF-A concentrations: Group1 (no VEGFA), Group2 (1 ng/ml VEGFA), Group3 (10 ng/ml VEGFA), Group4 (20 ng/ml VEGFA), Group5 (50 ng/ml VEGFA)
We used VEGF-A from R&D Systems, Minneapolis, MN for the stimulation. Media change and VEGF-A stimulation were done on days 0,2,4,6. Assay fixation was on day 7.
Staining: DAPI staining for nuclei, phalloidin staining for F-actin.
Notes
If you use the data, please cite: Beter, M., Abdollahzadeh, A., Pulkkinen, H. H., Huang, H., Orsenigo, F., Magnusson, P. U., ... & Laakkonen, J. P. (2023). SproutAngio: an open-source bioimage informatics tool for quantitative analysis of sprouting angiogenesis and lumen space. Scientific Reports, 13(1), 7279.
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Additional details
Funding
- IDENTIFYING LESION LANDSCAPE FOR PRECISION MEDICINE: GENETICS, SUBCLONALITY AND FUNCTIONAL COOPERATIVITY OF CELLS IN CONGENITAL VASCULAR ANOMALIES 321535
- Academy of Finland
- Gene, Cell and Nano Therapy Competence Cluster for the Treatment of Chronic Diseases (GeneCellNano) / Consortium: GeneCellNano 337120
- Academy of Finland
- GenomMed – Genomics and Mechanisms in Translational Medicine Doctoral Programme 740264
- European Commission
- IDENTIFYING LESION LANDSCAPE FOR PRECISION MEDICINE: GENETICS, SUBCLONALITY AND FUNCTIONAL COOPERATIVITY OF CELLS IN CONGENITAL VASCULAR ANOMALIES 328835
- Academy of Finland