Comparative analysis of keratinophilic fungi from the soils of Khardung and Khardung La (Ladakh), India

ABSTRACT

The present research work was undertaken to study the diversity of keratinophilic fungi from the unexplored soils of Khardung and Khardung La (Ladakh). This was done with a view to evaluate the relationship between the keratinophilic fungi and the keratinous material present in the soil. The soil samples examined from Khardung La were found to be 80% positive for keratinophilic fungi, whereas that of Khardung were 100% positive. A total of 29 keratinophilic species belonging to 13 genera were recovered by keratin bait technique from the soils of Khardung. However, from Khardung La, only 7 species belonging to 3 genera were recovered.  The recovered myco-keratinophiles consisted of 5 species each of Aspergillusand Fusarium, 4 species of Chrysosporium, 3 species of Paecilomyces, 2 species each of Acremonium, Gliocladium and Penicilliumand 1 species each ofBeauveria, Cladosporium, Embellisia, Emericella, Epicoccum, and Sagenomella.  Comparison of diversity indices showed a significant difference in species richness between the two sites i.e., Khardung (29) and Khardung La (7). The values of Shannon’s diversity index and Simpson’s diversity index were 3.27 and 0.96 respectively for Khardung, whereas these values were 1.88 and 0.84 respectively for Khardung La. Sorenson’s similarity index showed a value of 38.89% for these two sites, which indicates that some of the species present in Khardung La soils are also prevalent in Khardung soils.

Key words: Keratinophilic fungi, keratin, Khardung, Khardung la, Ladakh, soil.

REFERENCES

1. Kushwaha RKS. The genusChrysosporiumits physiology and biotechnological potential. In: Biology of dermatophytes and other keratinophilic fungi. Eds. RKS Kushwaha and J Guarro. RevistaIberoamericana de Micologia, Spain 2000; 66-76.
2. Filipello MV. Keratinophilic fungi: their role in nature and degradation of keratinic substrates. In: Kushwaha RKS and Guarro J, editors. Biology of dermatophytes and other keratinophilic fungi. RevistaIberoamericana de Mycologia, Spain 2000; 86-92.
3. Kunert J. Physiology of keratinophilic fungi. In: Kushwaha RKS and Guarro J, editors. Biology of dermatophytes and other keratinophilic fungi. RevistaIberoamericana de Mycologia, Spain 2000; 77-85.
4. Rippon JW. Clinical aspects of medically important conidial fungi. In: Cole GT, Kendrick EB, eds. Biology of conidial Fungi. New York: Academic Press, 198: 3-35.
5. Agnihotri SK, Agrawal SC. Keratinophilic fungi and related dermatophytes from the soils of Madhya Pradesh (India). Proc Nat AcadSci India 1989; 59: 193-196.
6. Deshmukh  SK . Isolation of dermatophytes and other keratinophilic fungi from soil of Mussoorie (India). Mykosen 1985; 28: 98-101.
7. Deshmukh SK. Keratinophilic fungi isolated from soils of Mumbai, India. Mycopathologia 1999; 146: 115-116.
8. Deshmukh  SK . Incidence of dermatophytes and other keratinophilic fungi in the glacier bank soils of the Kashmir Valley (India).  Mycologist 2002; 16: 165-167.
9. Kotwal S, Sumbali G. Incidence of myco-keratinophiles in cold arid soil at high altitude Khardung village of Ladakh, India. J Mycol Pl Pathol 2011; 41: 72-76.
10. Dixit AK, Kushwaha RKS. Keratinophilic fungi of Andaman Islands, India. Ind J Microbiol 1990; 30: 349-350.
11. Garg AK. Occurrence of keratinophilicChrysosporiumCorda in Indian soils. MycopathMycolAppl 1965; 29: 189-192.
12. Kaul S, Sumbali G. Keratinophilic fungi from poultry farm soil of Jammu, India.  Mycologist 2000; 14: 289-291.
13. Jandial S, Sumbali G. Diversity of keratinophilic fungi from the botanical garden soil of North Indian University of Jammu. Proc Nat AcadSci India 2011; 359-365.
14. Ramesh VM, Hilda A. Incidence of keratinophilic fungi in the soil of primary school and public parks of Madras city, India. Mycopathologia 1999; 143: 139-145.
15. Randhawa HS, Sandhu RS. A survey of soil inhabiting dermatophytes and related keratinophilic fungi of India. Sabouraudia 1965; 4: 71-79.
16. Vidyasagar GM, Narayan H, Shivkumar D. Keratinophilic fungi isolated from hospital dust and soils of public places at Gulbarga, India. Mycopathologia 2005; 159: 13-21.
17. Vanbreuseghem R.  Keratin digestion by dermatophytes; a specific diagnostic method.  Mycologia 1952; 44: 176-182.
18. Carmichael JW. Chrysosporium and some other aleuriosporicHyphomycetes. Can J Bot 1962; 40: 1137-1173.
19. Chabasse D. Taxonomic study of keratinophilic fungi isolated from soil and some mammals in France. Mycopathologia 1988; 101: 133-140.
20. Booth C. The genus Fusarium. CMI, Kew, Surrey, England; 1971; 1-237.
21. Raper EB, Fennel DI.  The genus Aspergillus. The Williams and Wilkins Co., Baltimore, USA; 1965; 132-575.
22. Gams W. Cephalosporiumlike Hyphomycetes. Hyphomycete Course, Sugadaira, Japan; 1997; 1-122.
23. Simpson EM.  Measurement of diversity.  Nature 1949; 163: 688.
24. Pielou FD.  Ecological Diversity. Wiley Interscience, New York 1975.
25. Krebs CJ. Ecological Methodology. Harper Collins Publishers, New York 1989; pp 654.
26. Mercantini R, Marsella R, Cervellati MC.  Keratinophilic fungi isolated from Antarctic soil.  Mycopathologia 1989; 106: 47-52.
27. Mercantini R, Marsella R, Moretto D, Finotti E. Keratinophilic fungi in the Antarctic environment.  Mycopathologia 1993; 122: 169-175.
28. Maghraby TA, GherbawyYAMH , Hussein MA . Keratinophilic fungi inhabiting floor dusts of student houses at the South Valley University in Egypt. Aerobiologia 2008; 24: 99-106.
29. Ali-Shtayeh MS. Keratinophilic fungi isolated from children’s sandpits in the Nablus area, West Bank of Jordan. Mycopathologia 1988; 103: 141-146.
30. Mitra SK, Sikdar A, Das P. Dermatophytes isolated from selected ruminants in India. Mycopathologia 1988; 142: 13-16.
31. Rippon JW. The pathogenic fungi and the pathogenic Actinomycetes. Phiadelphia, WB Saunders Co., 2^nd Edition; 1982; 154-248.
32. Abdel-Hafez SI, Moubasher AH, Barakat A. Keratinophilic fungi and other moulds associated with air-dust particles from Egypt. Folia Microbiol 1990; 35: 311-325.
33. Kaul S, Sumbali G. Keratinophilic fungi from feathers of Indian poultry birds. Mycologist 200b; 14: 13-15.