STUDIES OF THE ANTIMICROBIAL AND ANTIOXIDANT ACTIVITY OF GOLD NANOPARTICLES

Due to the multi-drug resistant bacteria's increased involvement in the spread of numerous diseases, the development of antibacterial medicines is attracting more and more attention. One of the novel and important materials to be produced as antibacterial agents in the realm of nanotechnology is metallic nanoparticles, particularly gold nanoparticles. Uncaria gambir Roxb leaf extract has been used to successfully create nanoparticles as a bio reducing agent and triethanolamine is used as a capping agent when a flavonoid molecule in the leaf extract reduces Au⁺³. The effects of concentrations of triethanolamine and hydrogen tetrachloroaurate (III) acid on the stability and size of nanoparticles were studied. The stability of colloidal gold was successfully maintained by the addition of triethanolamine 1% as a capping agent. 100ppm of hydrogen tetrachloroaurate (III) acid is present in nanoparticles. Analysis of X-Ray Diffraction exhibited peak patterns with a crystallite size of 32.52 nm that were in accordance with the metallic gold standard. Transmission Particle study using an electron microscope revealed that they were triangular, hexagonal, and spherical, with the smallest Particles were 7 nm in size. Gold nanoparticles that were created shown excellent antibacterial action against S. aureus and E coli. Gold nanoparticles made by synthesis were shown to be stable and antimicrobial, indicating they have the potential to be developed in applications in biomedicine