STUDY OF ANTIBIOTIC RESISTANCE PATTERN AND DETECTION OF MECA GENE IN STAPHYLOCOCCUS AUREUS ISOLATED FROM CLINICAL SAMPLES

ABSTRACT

Staphylococcus sp. is an important opportunistic pathogen responsible for a variety of diseases, ranging from minor skin infections to life-threatening systemic infections. The objective of the study was to determine antibiotic resistance pattern of Staphylococcus aureus isolated from clinical samples and to detect the presence of mecA gene in the isolates. 10 different Staphylococcus strains were isolated from various clinical samples such as urine, pus, etc. Antibiotic resistance pattern of the isolated strains were studied using seven different antibiotics. Presence of mecA gene was analysed using PCR technique. mecA gene was observed in 70 % of the isolated Staphylococcus strains. Amplification of mecA gene gives an insight into pharmaceutical aspects of designing new effective drugs for the treatment of methicllin resistant Staphylococcus sp.

Keywords: Staphylococcus sp., clinical samples, antibiotic resistance, mecA

REFERENCES

1. Archer, G. L., Niemeyer, D. M., Thanassi, J. A. & Pucci, M. J. 1994. Dissemination among staphylococci of DNA sequences associated with methicillin resistance. Antimicrobial Agents and Chemotherapy 38, 447–54.
2. Baddour, M. M., AbuElKheir, M. M., Fatani, A. J. 2007. Comparison of mecA polymerase chain reaction with phenotypic methods for the detection of methicillin-resistant Staphylococcus aureus. Curr Microbiol; 55: 473-9.
3. Cappuccino JG, Sherman N (1992) Biochemical activities of microorganisms. In: Microbiology, A Laboratory Manual. The Benjamin / Cummings Publishing Co. California, USA
4. Denton, M., O’Connell, B., Bernard, P., Jarlier, V., Williams, Z., & Henriksen, A. 2008. Antimicrobial Susceptibility of Staphylococcus aureus Causing Primary or Secondary Skin and Soft tissue Infctions in the Community in France, the UK and Ireland. Journal of Antimicrobial Chemotherapy , 586-588.
5. Farzana, K., & Hameed, A. 2006. Resistance Pattern of Clinical Isolates of Staphylococcus aureus against Five Groups of Antibiotics. Journal of Research (Science), 19-26.
6. Hrabak, J., Zemanova, A., Chudackova, E. 2010. Mobile genetic elements in the epidemiology og Bacterial resistance to antibiotics. Epidemol Microbiol Imunol;59:55-66
7. Huletsky, A., Giroux, R., Rossbach, V., et al. 2004. New real-time PCR assay for rapid detection of methicillin-resistant Staphylococcus aureus directly from specimens containing a mixture of staphylococci. J Clin Microbiol, 42(5), 1875-1884.
8. Ito, T., MA, X., Takeuchi, F., Okuma, K., Yuzawa, H., & Hiramatsu, K. 2004. Novel type V staphylococcal Cassette Chromosome mec driven by a novel cassette chromosome recobinase, ccrC. Antimicrob Agents Chemotherapy, 2637-2651.
9. Kloos, W. E. and K. H. Schleifer, 1981. The genus staphylococcus in the prokaryotes: A handbook on habitat, isolation and identification of bacteria, Vols. 1 and 2.
10. Magilner, D., Byerly, M. M., & Cline, D. M. (n.d.). 2008. The Prevalence of Community-Acquired Methicillin-Resistant Staphylococcus aureus (CA-MRSA) in Skin Abscesses Presenting to the Pediatric Emergency Department. North Carolina Medical journal.
11. Malhotra-Kumar, S., Haccuria, K., Michiels, M., et al. 2008. Current trends in rapid diagnostics for methicillin resistant Staphylococcus aureus and glycopeptide-resistant Enterococus species. J Clin Microbiol, 46(5), 1577-1587.
12. Obiazi, H. A. K., Nmorsi, O. P. G., Ekundayo, A. O., Ukwandu, N. C. D., 2007. Prevalence and antibiotic susceptibility pattern of Staphylococcus aureus from clinical isolates grown at 37 and 44OC from Irrua, Nigeria. Afr. J. Microbiol. Res. 1 (5): 57-60
13. Suzuki, E., Hiramatsu, K. & Yokota, T. 1992. Survey of methicillin-resistant clinical strains of coagulase-negative staphylococci for mecA gene distribution.Antimicrobial Agents and Chemotherapy 36, 429–34.