Phytochemical and Chromatographic Studies in the Leaves Extract of Acalypha Indica

ABSTRACT

Acalypha indica is herb found in tropical countries. This plant used traditionally for treats various diseases. In the present study we carried out phytochemical analysis and TLC profiling were performed on different solvent extractions like n-hexane, chloroform, ethyl acetate, acetone and methanol of Acalypha indica leaves extracts. Fresh matured leaves were collected and shade dried. The leaves powder was successively extracted with different solvents. This study involves the preliminary screening of phytochemical and the qualitative thin layer chromatographic separation of secondary metabolites from the leaves of Acalypha indica. TLC profiling of the Acalypha indica was carried out using sequential extracts of solvents with varying polarity; n-hexane, ethyl acetate and acetic acid respectively. Qualitative phytochemical analysis of this plant confirm the presence of various phytochemical like alkaloids, carbohydrates, glycosides, saponins, proteins, tannins, phenols, amino acids, and starch in their leaf extracts. The TLC chromatograms constituted different coloured phytochemical compounds with different Rf values. It can be conveniently used to evaluate the quality of different area samples. For TLC, new solvent system developed for the best separation of the phytoconstituents present in the extracts.

KEYWORDS: Acalypha indica, Phytochemicals, TLC, Plant extracts, Retention factor (Rf)

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