Indirect Spectrophotometric Method for Estimation of Irbesartan in Pure and in Pharmaceutical Dosage Form Using Oxidation and Reduction Reaction

ABSTRACT

Spectrophotometric method was developed for estimation of  Irbesartan ( IRB )  in pure form and in pharmaceutical preparations. The idea of our procedure is oxidation of  IRB with recognised excess amount of N-bromosuccinimide (NBS) in acidic medium and formerly remaining NBS is estimated by Rhodamine (RD) dye and then we measure the absorbance of remaining dye at 577 nm. A calibration curve was linear over the concentration range 1 –30 μg.ml-1 of IRB with correlation coefficient equal to  0.9991. The molar absorptivity were determined to be  4.499 × 104 L.mol-1.cm-1 and sandell's index value  0.0095 μg.cm-2. We are calculated the(LOD) = 0.469 μg.ml-1and (LOQ) = 1.982,.The our proposed method raped, sensitive and very selective and  has been applied to the estimation of  IRB  in obtainable dosage form. The recoverability and selectivity of the proposed method were confirmed by applying a standard addition procedure.