Published September 28, 2022
| Version 5.18 Ion reporter
Dataset
Open
NGS in the clinical microbiology settings
Authors/Creators
- 1. Rambam Health Care Campus
Description
This dataset contains bam files from 16s rRNA targeted NGS sequencing from a variety of clinical specimens. The study included 55 specimens from 51 patients. We compared targeted NGS to Sanger sequencing in clinical samples submitted for Sanger sequencing.
NGS: We sequenced the amplified V1-2 or V4 regions of 16s rRNA gene with Ion S5™ System (Thermo Fisher Scientific). Data were analyzed using the Ion Reporter bioinformatics Software pipeline (Thermo Fisher Scientific), using a threshold of 1000 mapped reads for designating significant pathogens. BAM files uploaded to the Ion Reporter were mapped to the Silva 138 SSU database.
Files
0078-92-PT_21-09_26.02.21_430.pdf
Files
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