Published September 12, 2022 | Version v1
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Study the effect of alkaloids isolated from Typha domingensis pers. fruit on hepatotoxicity induced by CCl4 in rats

Authors/Creators

  • 1. Chemistry Department, College of Science ,University of Basrah ,Basrah , Iraq
  • 2. Chemistry Department, College of Science, University of Thi-Qar, Al-Nasiriya,

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Abstract

The present study was carried out to investigate the hepatoprotective effect of alkaloids isolated from Typha domingensis pers. fruit against hepatotoxicity induced by carbon tetrachloride in rats. Alkaloids from Typha domingensis per .fruit were isolated, preliminary phytochemical tests were done and the appropriate alkaloid dose for rats was determined. Rats were divided into three groups: group I (control group) received the normal saline, group II received CCl4 only, and group III received both an alkaloid isolated, and CCl4. The biochemical markers: aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP), total protein, and lipid profile (cholesterol, triglycerides, HDL, and LDL) were determined. A histopathological study of the liver was conducted. The results showed that CCl4 significantly elevated the levels of AST, ALT, ALP, serum triglycerides, cholesterol, and LDL concentration as compared with the control group. While lower HDL and total protein were observed in the CCl4-treated group compared with the control group. The result also showed that alkaloid isolated reduced the rise in liver index ALT, AST, ALP and serum triglycerides, cholesterol, and LDL levels caused by CCl4. Alkaloid isolated increased HDL and total protein levels compared with the group treated with CCl4 only. At the same time, the histological study of liver tissue  proved that alkaloid isolation lessened the harm liver tissue caused by CCl4. The experiment demonstrates  that alkaloids isolated have a good hepatoprotective effect.The present study was carried out to investigate the hepatoprotective effect of alkaloids isolated from Typha domingensis pers. fruit against hepatotoxicity induced by carbon tetrachloride in rats. Alkaloids from Typha domingensis per .fruit were isolated, preliminary phytochemical tests were done and the appropriate alkaloid dose for rats was determined. Rats were divided into three groups: group I (control group) received the normal saline, group II received CCl4 only, and group III received both an alkaloid isolated, and CCl4. The biochemical markers: aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP), total protein, and lipid profile (cholesterol, triglycerides, HDL, and LDL) were determined. A histopathological study of the liver was conducted. The results showed that CCl4 significantly elevated the levels of AST, ALT, ALP, serum triglycerides, cholesterol, and LDL concentration as compared with the control group. While lower HDL and total protein were observed in the CCl4-treated group compared with the control group. The result also showed that alkaloid isolated reduced the rise in liver index ALT, AST, ALP and serum triglycerides, cholesterol, and LDL levels caused by CCl4. Alkaloid isolated increased HDL and total protein levels compared with the group treated with CCl4 only. At the same time, the histological study of liver tissue  proved that alkaloid isolation lessened the harm liver tissue caused by CCl4. The experiment demonstrates  that alkaloids isolated have a good hepatoprotective effect.

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