Figure 3–Figure Supplement 4 of the paper 'A Feed-forward Pathway Drives LRRK2 kinase Membrane Recruitment and Activation'

Raw immunoblotting and quantitation data for Figure 3–Figure Supplement 4 of the paper "A Feed-forward Pathway Drives LRRK2 kinase Membrane Recruitment and Activation" (Edmundo G. Vides, Ayan Adhikar, Claire Y. Chiang, Pawel Lis, Elena Purlyte, Charles Limouse, Justin L. Shumate, Elena Spínola-Lasso, Herschel S. Dhekne,  Dario R. Alessi, and Suzanne R. Pfeffer)

File descriptions:

Figure 3–Figure Supplement 4A
Fig.3.S4A_700(tRab10;tLRRK2)_High.tif - Licor scan (channel 700) showing total Rab10 and total LRRK2 blots; high contrast
Panel 1 - top left membrane
Panel 2 - top right membrane
Panel 3 - bottom left membrane
Panel 4 - bottom right membrane

Fig.3.S4A_700(tRab10;tLRRK2)_Low.tif - Licor scan (channel 700) showing total Rab10 and total LRRK2 blots; low contrast
Panel 1 - top left membrane
Panel 2 - top right membrane
Panel 3 - bottom left membrane
Panel 4 - bottom right membrane

Fig.3.S4A_800(pRab10;pLRRK2)_High.tif - Licor scan (channel 800) showing Rab10 pT73 and LRRK2 pS935 blots; high contrast
Panel 1 - top left membrane
Panel 2 - top right membrane
Panel 3 - bottom left membrane
Panel 4 - bottom right membrane

Fig.3.S4A_800(pRab10;pLRRK2)_Low.tif - Licor scan (channel 800) showing Rab10 pT73 and LRRK2 pS935 blots; high contrast
Panel 1 - top left membrane
Panel 2 - top right membrane
Panel 3 - bottom left membrane
Panel 4 - bottom right membrane

Fig.3.S4A_HA.tif - ChemiDoc Scan (ECL) showing HA blot

Figure 3–Figure Supplement 4B

Fig.3.S4B_700(tRab10;tLRRK2)_High.tif - Licor scan (channel 700) showing total Rab10 and total LRRK2 blots; high contrast
Panel 1 - top left membrane
Panel 2 - top right membrane
Panel 3 - bottom left membrane
Panel 4 - bottom right membrane

Fig.3.S4B_700(tRab10;tLRRK2)_Low.tif - Licor scan (channel 700) showing total Rab10 and total LRRK2 blots; low contrast
Panel 1 - top left membrane
Panel 2 - top right membrane
Panel 3 - bottom left membrane
Panel 4 - bottom right membrane

Fig.3.S4B_800(pRab10;pLRRK2)_High.tif - Licor scan (channel 800) showing Rab10 pT73 and LRRK2 pS935 blots; high contrast
Panel 1 - top left membrane
Panel 2 - top right membrane
Panel 3 - bottom left membrane
Panel 4 - bottom right membrane

Fig.3.S4B_800(pRab10;pLRRK2)_Low.tif - Licor scan (channel 800) showing Rab10 pT73 and LRRK2 pS935 blots; high contrast
Panel 1 - top left membrane
Panel 2 - top right membrane
Panel 3 - bottom left membrane
Panel 4 - bottom right membrane

Fig.3.S4B_HA.tif - ChemiDoc Scan (ECL) showing HA blot

Fig.3.S4_immunoblotting_numerical_data.xlsx - Excel sheet containing raw quatitation values from Licor scans as well as calculated pRab10/Rab10 ratios (values used in Figure 3C and 3D)