Published May 31, 2021
| Version v1
Project deliverable
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AfriDx D3.2 - Engineered Boon2-RT and accompanying expression and purification SOP
Description
Description and characterisation of Boon-RTX, a recombinantly expressed, engineered reverse transcriptase that catalyses RNA to DNA conversion. It has been fused with a silaffin-tag (R5) and mCherry reporter. This combination of proteins and tags allows for low-cost purification using silica and visual tracking through the prodcution process.
The protein also has a His-Tag to provide a secondary purification option.
Files include SOPs for Engineered Boon-RTX
- Protein Expression of Engineered Boon-RTX
- Silica Purification of Engineered Boon-RTX
Notes
The AfriDx project has been funded by EDCTP and the European Union's Horizon 2020 research and innovation program under grant number RIA2020EF - 2918.