Published July 25, 2022 | Version v1

Evaluation of tools for the taxonomical classification of viruses

  • 1. Doctorado en Ciencias, Centro de Investigación en Ciencias (CInC), Instituto de Investigación en Ciencias Básicas Aplicadas (IICBA), Universidad Autónoma del Estado de Morelos.
  • 2. Unidad Universitaria de Secuenciación Masiva y Bioinformática, Instituto de Biotecnología, Universidad Nacional Autónoma de México.
  • 3. Centro de Investigación en Enfermedades Infecciosas, Instituto Nacional de Enfermedades Respiratorias Ismael Cosío Villegas.
  • 4. Centro de Investigación en Ciencias, Universidad Autónoma del Estado de Morelos.
  • 5. 5Departamento de Genética del Desarrollo y Fisiología Molecular, Instituto de Biotecnología, Universidad Nacional Autónoma de México.

Description

50G (1942_50GL), 500G (1943_500GL) and 1000G (1752_1000GL) sets are simulated reads previously reported Tangherlini et al. [1], and represent large reads like contigs.

FISH-I (FISH_1_Q_sS_dup_HNoM_RbNoM), PB3(PB3_Q_sS_dup_HNoM_RbNoM), I5-8(ISA32_R1_Q_CDhit_HmNoM_RbNoM and ISA32_R1_Q_CDhit_HmNoM_RbNoM) and 121-1 (ISA44_R1_Q_CDhit_HmNoM_RbNoM and ISA44_R1_Q_CDhit_HmNoM_RbNoM) sets are real metagenomics reads previously reported Taboada et al. [2,3].

Eukaryotic, Prokaryotic, Unclassified, Bacterial and Human sets are simulated reads, generated by Grinder [4] software and represent short reads like those of Illumina technology.
 

Notes

1.Tangherlini M, Dell'Anno A, Zeigler Allen L, et al. Assessing viral taxonomic composition in benthic marine ecosystems: Reliability and efficiency of different bioinformatic tools for viral metagenomic analyses. Sci. Rep. 2016; 6:28428 2. Taboada B, Isa P, Gutiérrez-Escolano AL, et al. The geographic structure of viruses in the Cuatro Ciénegas Basin, a unique oasis in northern Mexico, reveals a highly diverse population on a small geographic scale. Appl. Environ. Microbiol. 2018; 84:1–25. 3. Taboada B, Morán P, Serrano-Vázquez A, et al. The gut virome of healthy children during the first year of life is diverse and dynamic. PLOS ONE 2021; 16:1–18. 4. Angly FE, Willner D, Rohwer F, et al. Grinder: A versatile amplicon and shotgun sequence simulator. Nucleic Acids Res. 2012; 40:e94-e94.

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