Published December 24, 2021
| Version v1
Dataset
Open
LotuS2: An ultrafast and highly accurate tool for amplicon sequencing analysis
Creators
- 1. Quadram Institute Bioscience & Earlham Institute
- 2. Earlham Institute
- 3. Quadram Institute Bioscience
- 4. Swedish University of Agricultural Sciences & University of Tartu
Description
Amplicon sequencing is an established and cost-efficient method for profiling microbiomes. However, many available tools to process this data require both bioinformatics skills and high computational power to process big datasets. Furthermore, there are only few tools that allow for long read amplicon data analysis. To bridge this gap, we developed the LotuS2 (Less OTU Scripts 2) pipeline, enabling user-friendly, resource friendly, and versatile analysis of raw amplicon sequences.
Notes
Please use the "TechnicalReplicate" column in the metadata to match the sample from each marker gene with its replicate.
Files
Files
(3.7 GB)
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md5:0bfe7c405cd598936fb86f12e8520f5d
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2.4 MB | Download |
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md5:fb341d11a1e0ec66fd2b9e6a4c9b270a
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2.5 MB | Download |
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md5:e727bd73aa6f1ab678a991e18fbe0c73
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2.5 MB | Download |
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md5:a02698e75c5b6df2dbbf9f368a67690d
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2.6 MB | Download |
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md5:efa84ea94ccf067c34410b85570aecb1
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2.6 MB | Download |
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md5:5f3cf44a66d8aaa1a2e19a0a84ce4089
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2.8 MB | Download |
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md5:eb790afbc15a47c89c2cbed01d65ee66
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2.4 MB | Download |
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md5:43cab147ed57b2f8fd6db0f87c1687a7
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2.5 MB | Download |
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md5:1c7714a8bc127144cb60c937bc0501f3
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5.5 MB | Download |
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md5:f84b86b688518a59321a09081ba8454c
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5.7 MB | Download |
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md5:4b3967f5d5f51ab7c808fa342dec5cfb
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8.4 MB | Download |
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md5:6672d2e8f76e944d5340cc3124ea7a09
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7.8 MB | Download |
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md5:db8145b8a9eef8f52fafebc03530b1fc
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6.6 MB | Download |
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md5:153f8ab0538012d92eb3335af00247e5
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7.1 MB | Download |
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md5:22064c64c9e007890cae211a609d1f04
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16.4 MB | Download |
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md5:25a6ce14fb6c74c258a9aab247fa5eda
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16.7 MB | Download |
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md5:941e770d479f56ba311a6416c11c5fbd
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4.0 MB | Download |
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md5:e93bc5239365f727c43216f25d9d3986
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4.2 MB | Download |
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md5:b2d82a40b91ae433b1e4caeb29ecc25f
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1.2 MB | Download |
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md5:2de95b3fba165283f6e11bc031872e09
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1.3 MB | Download |
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md5:3d50af3aa0edfb567200ea30d66f6d74
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6.1 MB | Download |
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md5:0e3e5a26ad63fd09e46ba72ee3c88cfc
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6.7 MB | Download |
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md5:c576b63a5c69c070612673055072cc76
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3.9 MB | Download |
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md5:245c9a3e5d6f62263d02011671a5e370
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4.0 MB | Download |
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md5:187d1af561c30166104876e7223eb2bd
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5.2 MB | Download |
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md5:4b7c1d7802b3eed59fc72714f6e02b93
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5.4 MB | Download |
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md5:563e105cdb975b74c78a1044f5a5a100
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1.2 MB | Download |
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md5:4d113b41299199a10f3f2249de3c2779
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1.3 MB | Download |
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md5:d63d468427f9f659c473bc709a539792
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3.7 MB | Download |
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md5:e8954d263c3a56921b88d46fb406cbf0
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3.7 MB | Download |
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md5:ff025a34398d63cd6edbcb3a09dc8405
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9.4 MB | Download |
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md5:425fa245e2e9c7c6d3356da4760968e5
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9.9 MB | Download |
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md5:e7b8493e2e68fba1b78ae95520b30026
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8.0 MB | Download |
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md5:30aadf5d0a296cc68ffb3a3bf0516572
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8.7 MB | Download |
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md5:d80086de4e16d30740fcde14694a751b
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9.4 MB | Download |
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md5:d54e50621b366a02359a85de7318301b
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9.7 MB | Download |
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md5:10609f468e79bbbd07c1e45a7362906d
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27.8 MB | Download |
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md5:34ff14fb1183ca0d36314292e489faba
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28.8 MB | Download |
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md5:52c99f16d010a29a4b7815d2c2c6867d
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4.4 MB | Download |
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md5:a7ea2b8f7683c476f653962c46b9df32
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4.2 MB | Download |
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md5:051229802f0e9e2ed6d650c56fe930ef
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2.3 MB | Download |
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md5:020ac504921463b486fda16df4b2b4d8
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2.4 MB | Download |
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md5:10ba7a79a9474fb990da40570e12f335
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11.0 kB | Download |
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md5:d22f7e260170e3c7f9b3141b0c820965
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13.6 kB | Download |
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md5:8d4c40c064a4d616a8d41f322ab151ff
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3.3 MB | Download |
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md5:a538b3b4080c8481f3edfd11205a85d7
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3.4 MB | Download |
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md5:cd85f844d0e9068089b97dac955dda6c
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1.1 MB | Download |
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md5:d1cb622f78449f3c127db8adc7fee3df
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1.1 MB | Download |
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md5:3119cb2a161f37e768d74045100adf16
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13.8 MB | Download |
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md5:fdfaef88a77141a3c5e5fe30d6a4f4ba
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15.7 MB | Download |
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md5:592e701be008d2ec7f4aade815ace18b
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16.7 kB | Download |
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md5:566971969c2e8ac83ac0fc9839c368e2
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19.7 kB | Download |
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md5:6bf7344058f995d6c997542555d06b77
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12.5 MB | Download |
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md5:a79a4a3d65c6c036d7aae88cc8b41d66
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13.3 MB | Download |
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md5:cdf68a53371e8c87ad85277041f424cf
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6.9 MB | Download |
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md5:8a4c0b507ff3240326e815e0903ac771
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7.1 MB | Download |
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md5:5ae24ad7334971242effd86ee92e6811
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4.5 MB | Download |
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md5:70dd4c39f13ab0be2da59ac19a5d7063
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4.5 MB | Download |
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md5:c8d83274448f11444eb16a96351a9670
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10.8 MB | Download |
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md5:acf0b48ab76bd3a674e5212009383943
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11.3 MB | Download |
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md5:1b5d104ecba7a3256219d20843153372
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4.8 MB | Download |
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md5:c48c4664464656900ec379121a03a9b2
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5.0 MB | Download |
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md5:7d26e08ffbb76504d5932ad12b14f025
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5.6 MB | Download |
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md5:a5b38ee3a7d29d62134b2ce5ae4159eb
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5.8 MB | Download |
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md5:64d469625b56a4b1042fda25b741f2cd
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5.6 MB | Download |
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md5:d34615753525c1f2c29437fdcca2eb26
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5.7 MB | Download |
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md5:fc1cc6fc874d6cfa3d06fb5cf75e90f0
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12.0 MB | Download |
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md5:8b631255cd33107dedb8ee4e1d1b5e5c
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12.4 MB | Download |
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md5:0acdd3bf5ff87b91774b935100b0442a
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9.6 MB | Download |
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md5:a6965ff38df326a28204ccac496632d2
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10.2 MB | Download |
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md5:224850d68ce9e6a27a8840b7b7344ab8
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2.0 MB | Download |
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md5:20d6f99157fa04a870a523b14c3cdb94
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2.1 MB | Download |
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md5:98e555b84b6d2a891a2f1e1c01ca81a3
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10.0 MB | Download |
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md5:694b5a2b1f211dbb3f8e4d5a440edbe0
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10.6 MB | Download |
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md5:8a3dbf7e488f49b4d2a8d3fc351a293e
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13.1 MB | Download |
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md5:9a47838c56aad3af4f600d0818ca213f
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13.9 MB | Download |
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md5:748e0eb366dcb9b33e1e7f9f5a08820a
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11.0 MB | Download |
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md5:b7495370ccfde2d5fbfa11c736e392ec
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11.9 MB | Download |
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md5:a33c272655b8a964c2fb6aa2515c7bf0
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2.4 MB | Download |
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md5:8f5b23f2427c21d62549d6167ccbee1c
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2.6 MB | Download |
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md5:6955e838470d94ff6d465cff7ea9f82c
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11.8 MB | Download |
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md5:9ca2a52cf610a1c69bc74a76ad4e5506
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12.4 MB | Download |
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md5:ced4e77b1e0bb7048e3864f76621a692
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2.3 MB | Download |
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md5:2731c4ef15f7eef2f12d0c4e6c26b78b
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2.5 MB | Download |
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md5:5d72275ea308869b814bd883f14b575e
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2.2 MB | Download |
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md5:721485743381a5982e5b7fa170845940
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2.4 MB | Download |
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md5:344c1b1ecd10b38fb28f9cdef97731cc
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1.1 MB | Download |
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md5:21d34d8a944306665ab07ca097613fda
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1.2 MB | Download |
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md5:7435bbaa565ee3c62aa25be7db9e1ced
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5.6 MB | Download |
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md5:d87b7ea52d0dd2fd53de4c9f668760f3
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5.9 MB | Download |
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md5:35cbc96d37f7a13383ec93bdd1057394
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1.6 MB | Download |
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md5:06955e2d2f149ec2d11c4576dd9bf036
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1.8 MB | Download |
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md5:8cdf61ff0ada82d9879391e857e0c84f
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11.6 MB | Download |
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md5:fe4acfcaaedd4a144f578b1572f91046
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11.3 MB | Download |
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md5:d683c611f7162744ce8108f99963d83c
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1.4 MB | Download |
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md5:a9f32f63a896aa75e6434b1ee7ae4df9
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1.5 MB | Download |
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md5:35108e40f312b8c4ed4635bc78070d8c
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7.7 MB | Download |
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md5:9b57786ba02932fddfe6df4087c1c749
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8.2 MB | Download |
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md5:fac94a962c0dc427dc35541f66bca5d7
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2.4 MB | Download |
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md5:98b6dcafb109b0a6814cb36361d842b3
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2.6 MB | Download |
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md5:9f89d47a6ef71201034f8a99078677e7
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2.5 MB | Download |
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md5:5d58603d8d629484924a49d64a391686
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2.6 MB | Download |
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md5:85fc5d4857708634605870a31d711ba3
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2.6 MB | Download |
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md5:9ae03895b1f3865099bcc25778945400
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2.8 MB | Download |
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md5:3215b91e82d39b84bedf10c0755ebf91
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2.4 MB | Download |
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md5:9c27dad79660de2edfebbef56f3a6ff0
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2.6 MB | Download |
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md5:e55b940edbd69db48926ad2710eab946
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5.6 MB | Download |
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md5:2e89b7ddbd15bb86f166dab9da1db72c
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5.7 MB | Download |
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md5:2748a3cb11e794ed1c74859b469317fc
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8.5 MB | Download |
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md5:4298399e66217a40c84a05110731c14e
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8.0 MB | Download |
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md5:b0c0bbfbc65b3f728593467425c68ffb
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6.8 MB | Download |
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md5:d9f3eb1fc5466df74ca6bd4425f58a03
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7.3 MB | Download |
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md5:ca32f8abf84c44ffbddedffbf31a9417
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16.5 MB | Download |
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md5:bdfbdd475d2dc904f98988839099651a
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17.0 MB | Download |
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md5:d9c92aa86d93c9533b9220fbb9a50a42
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4.0 MB | Download |
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md5:0aae14343391a28239beb5d9259cfbc5
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4.3 MB | Download |
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md5:ea1d5131e8c3d58601d08d82edca63b1
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1.2 MB | Download |
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md5:14a3a170be84ff264e4117374e5355f9
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1.3 MB | Download |
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md5:4063db385f0d42ff9bc80403bf44b947
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6.1 MB | Download |
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md5:eb3cb873e6eb93da181eae8a16f7506d
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6.7 MB | Download |
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md5:79ff31cff70b75ef4a442c332f1e5f98
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3.9 MB | Download |
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md5:7a87d3a906aa2ba5eccea7f6eab4b553
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4.0 MB | Download |
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md5:ddf43d0414db0d81c95ab91af45af910
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5.3 MB | Download |
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md5:7580fd07e469aef4f326fd273faa9c3d
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5.6 MB | Download |
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md5:b26f65c560c19c93d1029938a11e8ef3
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1.3 MB | Download |
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md5:c91593ec5aa6125c45cbd5e54d28a800
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1.3 MB | Download |
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md5:376e6d4adf64dcb9d7c910dc0dd60fe2
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3.8 MB | Download |
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md5:54e5a2a974b31a4a1e0d5cd1721f2c25
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3.8 MB | Download |
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md5:7c16d0213437c995148f11b64e68f370
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9.5 MB | Download |
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md5:2db905b176734c19133cce529b64ef73
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10.0 MB | Download |
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md5:4c416fec0a0fd92ebbbc1853fd210ac9
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8.0 MB | Download |
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md5:5a0e458385b8a7d6338d00cf5747b9bd
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8.6 MB | Download |
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md5:dca2527dfb525bcdb02eee8ed0f6b094
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9.5 MB | Download |
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md5:8214cc49da09e0191c081027035cc3f8
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9.9 MB | Download |
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md5:ad24c2d9fc16619c36cf15e1ff2f8ef7
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27.7 MB | Download |
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md5:a15ab00efb1b8136de2019c6aee3c56b
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28.9 MB | Download |
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md5:883da3b5972a13a0d426e906624cab1a
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4.3 MB | Download |
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md5:30bd7b99c9e08d0a1fc8288d464209ee
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4.3 MB | Download |
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md5:27edd6e48fc7fcb11a6d391855757614
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2.4 MB | Download |
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md5:8d87ebdcf9db8657eed3567a9ed5f905
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2.4 MB | Download |
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md5:b609887377e650fe95aafed01345b506
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13.3 kB | Download |
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md5:40518cc638e3d5a60f9692aa6d1b0c3c
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16.4 kB | Download |
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md5:60d1040d19b15dce71f80013b67c3f2d
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3.4 MB | Download |
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md5:de7fd279b8a673f83498d6b79c0a0158
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3.4 MB | Download |
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md5:1c5e62d24f167031a4ec441a041da452
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1.1 MB | Download |
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md5:9acc0cc6e33a31990612828c18019ebb
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1.1 MB | Download |
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md5:2e55673c5ce19dffe16554751d6cc274
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14.4 MB | Download |
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md5:2a474f61af288cb730549ae8f795675e
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16.3 MB | Download |
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md5:5be1726a254a307b83955cbb6daf5a8a
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17.1 kB | Download |
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md5:07fdc2fe7d29f246a37d4c837d3580c6
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20.1 kB | Download |
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md5:d5e7d81331314ef9985828526cbd2b8f
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12.7 MB | Download |
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md5:98bf1f9ee186ee76cbc434176b70ecda
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13.6 MB | Download |
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md5:9843f024a6932a617ad05a1e728c9fc8
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7.0 MB | Download |
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md5:ce6f35bcda7c5b14255b906e2f0d8c22
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7.3 MB | Download |
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md5:9d7bde2e101d0081570b9af925a89633
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4.5 MB | Download |
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md5:aa3798e4f67c8eda34177762726794ba
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4.6 MB | Download |
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md5:19e42445e28fe0e53ed97314e818c0fe
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10.8 MB | Download |
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md5:e610f25f37bb2148d9870cbb644d6465
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11.3 MB | Download |
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md5:5212c70d2249e10ee3427783a2d4c9cc
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4.8 MB | Download |
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md5:0bf401728f46f00cd319eecbb9f9d38a
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5.1 MB | Download |
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md5:f46912a9a0f04f82828e059553cca806
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5.8 MB | Download |
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md5:3b7b13d203e8e60974dd60058714c0e1
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6.0 MB | Download |
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md5:674c9029a8568a085691d326c6204984
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5.8 MB | Download |
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md5:d608e3488353f79195bfaa5afcd86d3a
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5.9 MB | Download |
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md5:ef5de700d4e26bfb257bc23d1d5946b2
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11.7 MB | Download |
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md5:a76b553abf12613fa6f19673a2b2565c
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12.1 MB | Download |
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md5:23eee0a4f2672edd8a9c00921a5c2f9d
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9.6 MB | Download |
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md5:d14f32e46188a576c8a62013822da21d
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10.2 MB | Download |
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md5:ad8e308ce7ee7eeb5ccd05d71d756584
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2.0 MB | Download |
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md5:1b945728eb33ba12deb31e7971b320b3
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2.0 MB | Download |
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md5:85b0d38bea09ac1822c71cc89374b1f6
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9.8 MB | Download |
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md5:c7899cb66d6411d974a7282083b82d72
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10.5 MB | Download |
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md5:3c70845be42fb0c72760e512a27ff808
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13.2 MB | Download |
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md5:855a066f6b162c992049d92642677c3a
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13.9 MB | Download |
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md5:dea6b958281372d61c2b5cc046224355
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11.0 MB | Download |
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md5:7a0ad673ac61632048e02e57aed461ac
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11.9 MB | Download |
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md5:25dbe1956540dd525ad0d44b14190f5a
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2.3 MB | Download |
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md5:21f1e65f88ff17f9b5b0b8015b581f03
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2.6 MB | Download |
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md5:2d6f8a1e7708f78eb0bd95c0ca179d24
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12.1 MB | Download |
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md5:c72bf3e58bdd2b0303e6fafb07d9e6e0
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12.7 MB | Download |
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md5:6ac3e7cf8b6469e63ecb919f6a6eeab3
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3.2 MB | Download |
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md5:2a1a9ea7dcf9116df5e87703ea09969d
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3.4 MB | Download |
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md5:8a86e13ecf898f3c797586fd911c2d2c
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2.3 MB | Download |
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md5:479a3efef459672c0a670d776405f51e
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2.4 MB | Download |
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md5:fa9b9033df70f213dc0dfe33c4d2d953
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2.3 MB | Download |
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md5:d152cecd8d9371bfe4575b0c22d75db3
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2.5 MB | Download |
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md5:c99244821553af0dc8151123d7b147ec
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1.1 MB | Download |
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md5:b275421b791e80a1d9e3ca0f757cc067
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1.2 MB | Download |
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md5:1ddc4cdabc90dfb0e85357b8ada4e40a
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5.8 MB | Download |
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md5:c5e35b3265c18995226750139f86a076
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6.1 MB | Download |
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md5:15b9088f62f18144a5f9afa2fdf6376b
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1.7 MB | Download |
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md5:cc6325e5ba4e525c4343ab5b23e6cf6a
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1.8 MB | Download |
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md5:cc27a2610a379b1b3f68551e5bbf3b00
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14.6 kB | Download |