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Published July 8, 2022 | Version Jul2022

Jul2022 reference datasets for Mitohelper

  • 1. University of Miami
  • 2. Mississippi State University

Description

Reference datasets (July 2022 update) for Mitohelper (https://github.com/aomlomics/mitohelper)

Mitohelper is a repository built to facilitate experimental design, alignment visualization, and reference sequence analysis in fish eDNA studies. Refer to our paper and Mitohelper's wiki for database construction pipeline.

I. Reference database files in tab-separated format, containing gene, taxonomy, and sequence information:

  • mitofish.all.Jul2022.tsv (748,772 records)
  • mitofish.12S.Jul2022.tsv (40,541 records)
  • mitofish.COI.Jul2022.tsv (265,763 records)

II. De-replicated QIIME 2-compatible 12S/12S+16S+18S rRNA reference datasets:

  • 12S-seqs-derep-uniq.qza 
  • 12S-tax-derep-uniq.qza 
  • 12S-16S-18S-seqs.qza 
  • 12S-16S-18S-tax.qza 

If you use Mitohelper, please cite:
Jean Lim, S, Thompson, LR. Mitohelper: A mitochondrial reference sequence analysis tool for fish eDNA studies. Environmental DNA. 2021; 00: 1– 10.  https://doi.org/10.1002/edn3.187

Notes

In the July version of Mitofish, 6,440 UNVERIFIED records were included. These were removed in mitohelper's dataset. In addition, 654 valid records were in old versions of Mitofish but not in the July version. These were not removed in mitohelper's dataset.

Files

Files (2.2 GB)

Name Size
md5:65d09c089ecf7432dd1b55e0c05393ef
106.8 MB Download
md5:dc31fb3f228073b527fafc4f0318d6fd
7.3 MB Download
md5:4f01315e843e8251f3ca4145249bc611
9.7 MB Download
md5:184f6f6aa4bb3b091658bc3b1515cab4
511.5 kB Download
md5:1dd8e244195a3917cf50aa0b6b59eb30
218.1 MB Download
md5:57d90c83d81adbfb2e2d2c3dc50da556
1.3 GB Download
md5:de6c163ae1bfcf0344d2b7b412edd6ee
506.3 MB Download