Published June 13, 2022 | Version 1.0
Dataset Open

Slimfield: Escherichia coli DNA repair proteins (RecA-mGFP and RecB-sfGFP)

Creators

  • 1. University of York

Description

Imaging modality / instrument: Brightfield + Slimfield

Image format: OME TIFF (16 bit) + MicroManager metadata files

Microscope settings:

488 nm triggered excitation; split detection, cropped to GFP or RFP/GFP (left/right) channels; 3 ms/frame laser exposure; Photometrics Prime95b CMOS

Samples and acquisitions:

Fluorescent fusions in live E.coli cells.  MMC = mitomycin C

No. fields of view

MMC-

MMC+ (0.5 ug/ml 3h)

RecA-mGFP

7

15

RecB-sfGFP

17

21

MG1655 control

10

10

Approx. size before/after compression: 21 GB / 7 GB

Files

RecAB raw Slimfield data.zip

Files (7.1 GB)

Additional details

Funding

Pushing proteins off DNA - how do helicases unwind protein-coated DNA? BB/P000746/1
UK Research and Innovation
Replication repair in real life: analysing how broken DNA replication machines are rebuilt inside cells. BB/N006453/1
UK Research and Innovation
Biological physics of protein clustering in epigenetic memory and transcriptional control EP/T002166/1
UK Research and Innovation

References

  • "RecA and RecB: probing complexes of DNA repair proteins with mitomycin C in live Escherichia coli with single-molecule sensitivity", Alex L. Payne-Dwyer, Aisha H. Syeda, Jack W. Shepherd, Lewis Frame, Mark. C. Leake bioRxiv 2021.10.04.463068; doi: https://doi.org/10.1101/2021.10.04.463068