Dataset Open Access

Slimfield: Escherichia coli DNA repair proteins (RecA-mGFP and RecB-sfGFP)

Alex L Payne-Dwyer; Aisha H Syeda; Mark C Leake

Imaging modality / instrument: Brightfield + Slimfield

Image format: OME TIFF (16 bit) + MicroManager metadata files

Microscope settings:

488 nm triggered excitation; split detection, cropped to GFP or RFP/GFP (left/right) channels; 3 ms/frame laser exposure; Photometrics Prime95b CMOS

Samples and acquisitions:

Fluorescent fusions in live E.coli cells.  MMC = mitomycin C

No. fields of view

MMC-

MMC+ (0.5 ug/ml 3h)

RecA-mGFP

7

15

RecB-sfGFP

17

21

MG1655 control

10

10

Approx. size before/after compression: 21 GB / 7 GB

Files (7.1 GB)
Name Size
Open Data Proforma Slimfield E. coli repair proteins (RecA-mGFP and RecB-sfGFP).docx
md5:3e06c5f081684ebc0b4bd2083d039b3a 		15.8 kB Download
RecAB raw Slimfield data.zip
md5:3bb5d51c1656a1946df61276b7e7b805 		7.1 GB Download

  • "RecA and RecB: probing complexes of DNA repair proteins with mitomycin C in live Escherichia coli with single-molecule sensitivity", Alex L. Payne-Dwyer, Aisha H. Syeda, Jack W. Shepherd, Lewis Frame, Mark. C. Leake bioRxiv 2021.10.04.463068; doi: https://doi.org/10.1101/2021.10.04.463068

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Publication date:
June 13, 2022
DOI:
10.5281/zenodo.6639101

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10.5281/zenodo.6639101

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Keyword(s):
DNA repair single-molecule imaging bacteria Slimfield fluorescence microscopy
Grants:
UK Research and Innovation:
  • Pushing proteins off DNA - how do helicases unwind protein-coated DNA? (BB/P000746/1)
  • Replication repair in real life: analysing how broken DNA replication machines are rebuilt inside cells. (BB/N006453/1)
  • Biological physics of protein clustering in epigenetic memory and transcriptional control (EP/T002166/1)
License (for files):
Creative Commons Attribution 4.0 International

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Version 1.0 10.5281/zenodo.6639101 Jun 13, 2022
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