Use of traC gene to type the incidence and distribution of pXFAS_5235 plasmid-bearing strains of X. fastidiosa subsp. fastidiosa ST1 in Spain

Figure S1: Agarose gels showing traC gene amplification for selected X. fastidiosa strains from the collection at the Instituto de Agricultura Sostenible (IAS-CSIC) using ND116-pRIV5-F1/ND117-pRIV5-R1 primer pair (expected amplicon size of 521bp). PAC = Positive amplification control contained boiled cells of IVIA5235 strain. NAC= Negative amplification control contained DNA from a Xf strain known not to harbor the traC gene. L= 10 Kb GeneRuler™ DNA Ladder Mix (Thermo Scientific™); Figure S2:  Alignments of all traC sequences obtained in this study and those retrieved from GenBank using MAFFT. single nucleotide polymorphisms are indicated in relation to the consensus sequence. The subspecies of the Xf strain is indicated between brackets (F= fastidiosa, M= multiplex, P= pauca, M= morus; S= sandyi, S/M= sandyi/morus)