Spectral flow cytometric tracking of mitochondria transfer in adipose tissue by age and diet
Description
Unmixed fcs files for the stromal vascular fraction of epididymal (eWAT), inguinal (iWAT), and brown adipose tissue (BAT) in mtD2F/+AdipoqCre+/- and different ages or diets.
For a breakdown of samples and code, please see: https://github.com/ncborcherding/mtD2
Antibody Panel:
|
Laser |
Channel |
Antigen |
Fluorophore |
Final Dilution Factor |
Vendor |
Clone |
Catalog Number |
|
Violet |
V1 |
SiglecF |
BV 421 |
1:400 |
BD Biosciences |
E50-2440 |
562681 |
|
V2 |
CD44 |
Super Bright 436 |
1:300 |
eBioscience |
IM7 |
62-044-180 |
|
|
V3 |
CD11b |
Pacific Blue |
1:300 |
BioLegend |
M1/70 |
101224 |
|
|
V5 |
IL-33R⍺-biotin |
n/a |
1:300 |
BioLegend |
DIH9 |
145308 |
|
|
V5 |
Streptavidin (2°) |
BV 480 |
1:300 |
BD Horizon |
n/a |
564876 |
|
|
V7 |
MHC-II (I-A/I-E) |
BV 510 |
1:300 |
BioLegend |
M5/114.15.2 |
107636 |
|
|
V8 |
Ly6C |
BV 570 |
1:400 |
BioLegend |
HK1.4 |
128030 |
|
|
V10 |
KLRG1 |
BV 605 |
1:300 |
BioLegend |
2F1/KLRG1 |
138419 |
|
|
V11 |
F4/80 |
BV 650 |
1:300 |
BioLegend |
BM8 |
123149 |
|
|
V13 |
Ly6G |
BV 711 |
1:300 |
BioLegend |
1A8 |
127643 |
|
|
V14 |
CD3 |
BV 750 |
1:300 |
BioLegend |
17A2 |
100249 |
|
|
V15 |
CD206 |
BV 785 |
1:200 |
BioLegend |
C068C2 |
141729 |
|
|
Blue |
B1 |
mtDendra2 |
n/a |
n/a |
n/a |
n/a |
n/a |
|
B2 |
TCRb |
AF 488 |
1:300 |
BioLegend |
H57-597 |
109215 |
|
|
B3 |
CD8 |
Spark Blue 550 |
1:300 |
BioLegend |
53-6.7 |
100780 |
|
|
B8 |
CD45 |
PerCP |
1:200 |
BioLegend |
30-F11 |
103130 |
|
|
B9 |
CD71 |
PerCP-Cy5.5 |
1:200 |
BioLegend |
RI7217 |
113816 |
|
|
B10 |
NKp46 |
PerCP-eFluor710 |
1:200 |
eBioscience |
29A1.4 |
46-3351-82 |
|
|
Green |
YG1 |
TCRgd |
PE |
1:300 |
BioLegend |
GL3 |
118108 |
|
YG3 |
CD64 |
PE/Dazzle 594 |
1:300 |
BioLegend |
X54-5/7.1 |
139320 |
|
|
YG4 |
CD4 |
PE/Fire 640 |
1:300 |
BioLegend |
GK1.5 |
100482 |
|
|
YG5 |
CD62L |
PE-Cyanine5 |
1:300 |
BioLegend |
MEL-14 |
104410 |
|
|
YG7 |
CD11c |
PE-Cyanine5.5 |
1:300 |
eBioscience |
N418 |
35-0114-82 |
|
|
YG9 |
CD25 |
PE/Cyanine7 |
1:200 |
BioLegend |
PC61 |
102016 |
|
|
Red |
R1 |
CD9 |
APC |
1:300 |
BioLegend |
MZ3 |
124812 |
|
R2 |
CD127 |
AF 647 |
1:200 |
BioLegend |
A7R34 |
135020 |
|
|
R3 |
CD19 |
Spark NIR 550 |
1:300 |
BioLegend |
6D5 |
115568 |
|
|
R4 |
CD90.2 |
AF 700 |
1:300 |
BioLegend |
30-H12 |
105320 |
|
|
R5 |
CD5 |
BUV 737 |
1:300 |
BD Horizon |
53-7-3 |
612809 |
|
|
R6 |
Viability dye |
Zombie NIR |
1:1,000 |
BioLegend |
n/a |
423106 |
|
|
R7 |
NK1.1 |
APC/Fire 750 |
1:300 |
BioLegend |
PK136 |
108752 |
|
|
R8 |
B220 |
APC/Fire 810 |
1:300 |
BioLegend |
RA3-6B2 |
103278 |
Files
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