ACTIVITY OF GLUTATION SYSTEM OF ERYTHROCYTES UNDER CONSUMPTION OF ENERGY DRINK

The glutathione system forms the functional basis of the erythrocyte antioxidant defense system. The aim of this study was to investigate the effect of energy on the activity of the glutathione system of erythrocytes, as it plays an important role in protection against oxidative stress. The study was conducted using male Wistar rats, which were kept in the vivarium under appropriate lighting conditions, temperature, humidity and standard diet. All animals had free access to feed (based on daily requirements) and water (based on 20 ml of water per rat per day). The amount of standard feed consumed for laboratory animals was determined by its residue in the feeder. Control over the growth and development of animals was performed by weighing them at the beginning and at the end of the experiments. The experimental animals were divided into five groups: 1st group – received drinking water (control group); 2nd group – received an energy drink for a month and the collection of material was carried out on the 1th day at the end of the experiment; 3rd group – received an energy drink for a month and the collection of material was carried out on the 10th day at the end of the experiment; 4th group – received an energy drink for a month and the collection of material was carried out on the 20th day at the end of the experiment; 5th group – received an energy drink for a month and the collection of material was carried out on the 30th day at the end of the experiment. The state of the antioxidant system was assessed by the activity of the glutathione system, namely glutathione peroxidase (EC 1.11.1.9), glutathione reductase (EC 1.8.1.7) and glutathione transferase (EC 2.5.1.18. Under the conditions of energy drink consumption, inhibition of glutathione peroxidase, glutathione reductase and glutathione-S-transferase activity was observed in the II, III, IV and V experimental groups in comparison with the control group (I). The obtained results indicate a violation of the enzymatic link of antioxidant protection of erythrocytes of animals that consumed energy drink, which can lead to disruption of homeostasis of the body as a whole.