Published May 4, 2022 | Version v1
Dataset Restricted

Chloroplast motion under dim light conditions

Authors/Creators

  • 1. University of Amsterdam

Description

Elodea densa was kept in an aquatic culture. Ambient light conditions were applied 1h before image acquisition. 
Imaging preparation: a low-light adapted leaf was detached and placed between two glass slides.
Lower cell layer is displayed. 
Microscopy: Bright field microscopy was performed with a Nikon TI2 microscope using a halogen light source and a red-light 620nm cut-on wavelength filter at low light intensities of  7.2 W/cm^2 in combination with a Photometrics BSI Express sCMOS camera with high quantum yield to enable imaging every 1s with a $63\times$ water-immersion objective (NA=1.2) and pixel resolution of 0.1µm/px. 

Description:
Chloroplast.zip:
Contains ome.tiff files for 3600 frames of chloroplast motion (total duration 1h)
The dense chloroplasts (dark circular objects) inside the plant cells move by actin-mediated propulsion mechanism. 

211013_CP_rawLabelMeasures.csv:
Analysis of segmented data (3600 frames) 

211013_CP_tracks_vel.csv:
Trajectories (with velocities) constructed by trackpy from center-of-mass positions of the 211013_CP_rawLabelMeasures.csv. 
Velocities where calculated using the first derivative of 3rd order Savitzky-Golay filtered data (Kernel width 11s). 



 

Files

Restricted

The record is publicly accessible, but files are restricted. Log in to check if you have access.