Published August 21, 2022
| Version v1
Journal article
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Cyani HAP2 structure compared to Cyani HAP2 monomeric and trimeric structures predicted by Alphafold
Authors/Creators
- 1. Department of Biological Chemistry and Molecular Pharmacology and Harvard Medical School, Boston, MA, USA
Description
To obtain a model of the postfusion state of Cyani HAP2, we used Alphafold 2 Multimer. For validation, we first predicted a monomeric structure by Alphafold 2 ColabFold. Compared to the Cyani crystal structure, the model was remarkably accurate, contained the interim β-sheet, and had Cα atom RMSDs of 0.5 to 0.6 Å for individual D1, D2, and D3 domains over all atoms and an RMSD of 3.4 Å over all 466 residues of the prefusion state.