Technical note Open Access

Production of Recombinant α Synuclein Monomers and Preformed Fibrils (PFFs)

Maneca, Destiny-Love; Luo, Wen; Krahn, Andrea; Del Cid Pellitero, Esther; Shlaifer, Irina; Nicouleau, Michael; Pimentel, Luisa; Beitel, Lenore K.; Rao, Trisha; Durcan, Thomas M.

This protocol describes how to:
• Produce and purify recombinant human and mouse α-synuclein in monomeric form from transformed bacteria
• Generate α-synuclein preformed fibrils (PFFs) from recombinant monomeric α-synuclein
• Fluorescently label α-synuclein PFFs

Version 3.0 now available

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Publication date:
April 9, 2022
DOI:
10.5281/zenodo.6430401

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Keyword(s):
alpha-synuclein protein expression bacteria GST-tag Preformed fibrils (PFFs)
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Creative Commons Attribution 4.0 International

Versions

Version V3.0 10.5281/zenodo.6430401 Apr 9, 2022
Version V2.0 10.5281/zenodo.3738335 Aug 21, 2019
Version V1.0 10.5281/zenodo.3372888 Aug 21, 2019
Cite all versions? You can cite all versions by using the DOI 10.5281/zenodo.3372887. This DOI represents all versions, and will always resolve to the latest one. Read more.

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