Published April 12, 2022 | Version v1
Dataset Open

Microbial diversity in streams draining proglacial floodplains

  • 1. River Ecosystems Laboratory, Ecole Polytechnique Fédérale de Lausanne, Switzerland

Description

This dataset contains X files: 

16S rRNA data:

  • 16S_table.csv: Absolute abundance for each ASV. Absolute abundance were obtaines by using the relative abundance form read count and multiply it by the bacterial abundance measured through flowcytometer. ASV are rows and samples are columns. 
  • 16S_Taxonomy.csv: The taxonomy obtained for each ASV. 
  • 16S_Metadata.csv: Information on the samples ( Glacier, date, longitude, latitude, stream type,...) 

18S rRNA data: 

  • 18S_otu_table.csv: Read count for each OTU. 18S ASVs were clustered into operational taxonomic units (OTUs) using a 97% identity threshold using the vsearch de novo clustering method implemented in qiime2.
  • 18S_Taxonomy_NEW.csv: The taxonomy obtained for each OTU, updated with SILVA update in October 2021. 
  • 18S_Metadata: Information on the samples ( Glacier, date, longitude, latitude, stream type,...) 

Water physiochemical parameter 

  • water_data.csv : [DOC, Na+, Mg2+, K, Ca2+, Cl-, SO42-,NH3, NOx, PO4, NO2, SRP, Alkalinity] are in ppb (mg/L) 
    [Temp] (Temperature) is in °C
    [Conductivity] is in uS/cm 
    [Turbidity] is in NTU 
    dD_H: water stable isotope of deuterium
    d18_16: water stable isotope Oxygen 

Sediment biomass data

  • Biomass_data.csv: 
    Chla (chlorophyll-a concentration) is in ug Chla / g of DM (dry mass) 
    Bacterial_abundance: Number of bacteria counted by flow cytometer 
    BCP: Bacterial carbon production is in ng C g-1DM h-1
    EPS: Extracelluar Polymeric substance is in glucose-equivalent g-1DM

Co-occurnece Network anaylsis - all file are in a subfolder /Network

  • Raw files to be pre-process : 
    • GFS_Fungi_links.csv, GFS_Fungi_nodes.csv, Trib_Fungi_links.csv, Trib_Fungi_nodes.csv
  • Pre-processed file used for plotting:
    • GFS_links_edit.csv, GFS_node_edit.csv, Trib_links_edit.csv, Trib_node_edit.csv,
       

Files

data.zip

Files (7.4 MB)

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